Tsuneo Takemaru

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Twenty-four sporeless mutants were isolated from an Amut Bmut strain (mutant in the incompatibility factors) of the basidiomycete Coprinus cinereus. All the sporeless mutations were recessive to the wild type. These mutants and a previously isolated recessive sporeless strain, N2-7 (Kanda and Ishikawa 1986) were crossed with a wildtype strain. An F1 random(More)
Meiotic events in the sporeless mutant, NG107, and in the wild-type dikaryon of the basidiomyceteCoprinus macrorhizus were studied comparatively with light and electron microscopes in order to determine precisely which meiotic process was blocked in the mutant. In most basidia of the mutant, when incubated at 28 C, meiosis stopped at meta-anaphase I(More)
Cell-free extracts and cell wall autolysates prepared from the stipes of basidiocarp ofCoprinus cinereus were examined for (1→3)-β-glucanase activities. Gel filtration revealed two major peaks and a minor one of (1→3)-β-glucanases in both of the preparations, the former ones being designated as glucanase I and glucanase II. Glucanase I with a molecular(More)
The dikaryon #5026 (A2B2)+#5132 (A7B7) of the basidiomyceteCoprinus macrorhizus was treated with ultraviolet light (UV),N-methyl-N′-nitro-N-nitrosoguanidine (NG), and 5-bromouracil (BU) in order to induced developmental variations in fruiting. Among a total of 11,383 isolates, 742 were homokaryons. Fruiting test was made with the remaining 10,641 dikaryotic(More)
Temperature-sensitive variants defective in hyphal growth of the basidiomyceteCoprinus cinereus were isolated from oidia treated by UV orN-methyl-N′-nitro-N-nitrosoguanidine. In 23 out of 27 variant strains isolated, the temperature-sensitive variation was shown to be due to recessive single-gene mutation. All of the 23 mutants exhibited apparent(More)
The production of NADP-dependent glutamate dehydrogenase was initiated at the stage of first meiotic prophase in pileus cells but not in stipe cells of dikaryotic and monokaryotic fruiting bodies in Coprinus macrorhizus. The production of chitinase and glucanases assayed with laminarin and lichenan was observed after the completion of meiosis only in pileus(More)
In total, 404 variants resistant to the antimicrotubule agent benomyl were isolated from UV-irradiated oidia of the basidiomyceteCoprinus cinereus. Part of the variants showed, in addition to benomyl resistance, heat sensitivity or heat dependence. Fifteen variants selected on the basis of different phenotypes were subjected to further analyses. All of the(More)
Chitin microfibrils are a major component of the walls of stipe cells of the mushroomCoprinus cinereus. They occur as shallow helices, which may be right-or left-handed, with about two thirds being left-handed. The sense of the helicity is constant throughout one cell, but cells with both senses are found in the same stipe. This shallow helicity is the same(More)
Crude extracts of mycelia and basidiocarp primordia in the basidiomycete Coprinus cinereus were resolved on sodium dodecyl sulfate--polyacrylamide gels, and ubiquitin and several proteins were detected by immunoblotting with anti-ubiquitin antibody. The molecular masses of the proteins detected were 30,900, 28,600, 27,800, 26,300, 22,500, and 15,400(More)
Four recessive primordiumless mutants from anAmut Bmut strain ofCoprinus cinereus were crossed with wild-type strain. Segregation analysis of F1-progeny and complementation test showed that these mutations were controlled by 4 single recessive genes. All these genes were unlinked to both theA andB factors.