Tsuguhiko Nakai

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Lymphocytes from bursa of Fabricius and thymus of chickens were purified and separated into the three cell subsets--T, B, and null cells--by the techniques of nylon fiber columns and cytotoxicity tests. The in vitro susceptibility of the fractionated lymphocytes to a virulent strain of infectious bursal disease virus (IBDV) was studied by using(More)
Ca2+ regulates a variety of cellular mechanisms in vascular cells as well as in platelets. Nicorandil interacts with the intracellular Ca2+-activated processes in vascular smooth muscle cells, while Ca2+ channel blockers such as verapamil and diltiazem block voltage-dependent Ca2+ channels. The effects of nicorandil are due to the hyperpolarization of the(More)
Variations of B lymphocytes bearing surface immunoglobulin (SIg) M [SIg(M)] and G [SIg(G)] were studied in the spleen and peripheral blood of chickens infected with infectious bursal disease virus (IBDV). The proportion of SIg-bearing B lymphocytes and SIg(M)- and SIg(G)-bearing B cells in chickens infected at one day of age decreased from 1 week(More)
Proliferation of vascular smooth muscle cells (VSMC) has been shown to play a key role in the atherosclerotic lesions. It has been demonstrated that serum-derived peptidic growth factors, such as insulin, platelet-derived growth factor (PDGF), or epidermal growth factor (EGF), provide mitogenic signals in VSMC and that the interplay of Ca2+ and other(More)
In the present study, the effects of maternal ethanol intake during pregnancy on microsomal cytochrome contents and on enzyme activities in the developing fetus were investigated. Ethanol intake to pregnant females did not affect the maternal body weight or number of fetuses, but tended to reduce the mean fetal body weight, especially the fetuses from a(More)
Rat liver parenchymal cell binding, uptake, and proteolytic degradation of rat ‘Z51-labeled high density lipoprotein (HDL) subfraction, HDL, (1.110 < d < 1.210 g/ml), in which apo-A-I is the major polypeptide, were investigated. Structural and metabolic integrity of the isolated cells was verified by trypan blue exclusion, low lactic dehydrogenase leakage,(More)
To investigate the effect of leukocyte and platelet depletion on reperfusion injury using a leukocyte-platelet removal filter (LRF), several cardiovascular variables were examined in the rabbits during ischemia followed by reperfusion. The rabbits underwent cytoapheresis with LRF (n=S) and were compared with controls without LRF (n=S). LRF was composed of a(More)
The isolated small bowel from duodenojejunal junction to ileocecal junction with intact SMA and SMV was perfused with Krebs-Ringer solution intraluminally in rat. After all the branches from aorta and portal vein other than SMA and SMV were ligated, aorta and portal vein were cannulated and perfused with Krebs-Dextran solution. The isolated perfused bowel(More)