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Lysostaphin Lysibody Leads to Effective Opsonization and Killing of Methicillin-Resistant Staphylococcus aureus in a Murine Model
- Assaf Raz, A. Serrano, Maneesha Thaker, Tricia Alston, V. Fischetti
- BiologyAntimicrobial Agents and Chemotherapy
- 23 July 2018
The first example of a lysibody containing the binding domain from a bacteriocin, lysostaphin, is described, which is a promising solution for opsonic antibodies that may be used passively to both treat and prevent infection by drug-resistant pathogens.
Comparison of Two High-Throughput Reverse Transcription-PCR Systems for the Detection of Severe Acute Respiratory Syndrome Coronavirus 2
The diagnostic performance of two high-throughput systems, cobas 6800 and Panther Fusion, and their associated RT-PCR assays, with a collection of 389 nasopharyngeal specimens imply that the two platforms can be considered comparable in terms of their clinical performance.
Gastrointestinal pathogen colonization and the microbiome in asymptomatic kidney transplant recipients
- L. Westblade, M. Satlin, J. Lee
- MedicineTransplant infectious disease : an official…
- 10 September 2019
In kidney transplant recipients, gastrointestinal (GI) pathogens in feces are only evaluated during diarrheal episodes. Little is known about the prevalence of GI pathogens in asymptomatic…
Gut microbiota profiles and fecal beta‐glucuronidase activity in kidney transplant recipients with and without post‐transplant diarrhea
The data reveal post‐transplant diarrhea as a complex phenomenon with decreased gut microbial diversity and commensal gut organisms, suggesting fecal β‐glucuronidase activity could be a novel biomarker for gastrointestinal‐related MMF toxicity.
Streptococcus pyogenes Sortase Mutants Are Highly Susceptible to Killing by Host Factors Due to Aberrant Envelope Physiology
Unanticipated effects of deletion of Streptococcus pyogenes sortase A lead to accumulation of sorting intermediates, particularly at the septum, altering cellular morphology and physiology, and compromising membrane integrity, suggesting that inhibition of sortase by small-molecule inhibitors could similarly lead to the rapid elimination of pathogens from an infected host.
Comparison of Two High-Throughput Reverse Transcription-Polymerase Chain Reaction 1 Systems for the Detection of Severe Acute Respiratory Syndrome Coronavirus 2 2
This work analyzed the diagnostic performance of two high-throughput systems: cobas 6800 and Panther Fusion, and their associated RT-PCR assays with a collection of 389 nasopharyngeal specimens to imply both platforms can be considered comparable in terms of their clinical performance.
Lysibodies are IgG Fc fusions with lysin binding domains targeting Staphylococcus aureus wall carbohydrates for effective phagocytosis
- Assaf Raz, A. Serrano, V. Fischetti
- BiologyProceedings of the National Academy of Sciences
- 20 April 2017
It is demonstrated that binding domains from autolysins and lysins can be fused to the Fc region of human IgG, creating a fully functional homodimer (or “lysibody”) with high-affinity binding and specificity for carbohydrate determinants on the bacterial surface.
Variations in the multimerization region of the Helicobacter pylori cytotoxin CagA affect virulence.
It is demonstrated that, following 18 h, the CagA-THE AUTHORS-transfected cells were less adherent compared with the CAgA-WW transfecting cells, which suggest that different CM motif types may affect CAGA virulence.