Tran Duc Thanh

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Green microalga Ankistrodesmus convolutus Corda is a fast growing alga which produces appreciable amount of carotenoids and polyunsaturated fatty acids. To our knowledge, this is the first report on the construction of cDNA library and preliminary analysis of ESTs for this species. The titers of the primary and amplified cDNA libraries were 1.1 × 106 and(More)
The availability of highly active homologous promoters is critical in the development of a transformation system and improvement of the transformation efficiency. To facilitate transformation of green microalga Ankistrodesmus convolutus which is considered as a potential candidate for many biotechnological applications, a highly-expressed native promoter(More)
A transect from the Tomoe River Mouth through Shimizu Port to Suruga Bay, Japan, was examined between 2005 and 2009 to reveal the population dynamics of Crenarchaeota and Euryarchaeota in an estuary environment. Crenarchaeota tended to increase in abundance in waters deeper than 100 m compared with Euryarchaeota, and comprised 11% of total direct counts.(More)
The majority of Hevea (Hevea brasiliensis Muell. Arg.) genetic resource in Vietnam derived from the IRRDB'81 germplasm collected in the Amazonian habitats of the genus. Random amplified polymorphic DNA (RAPD) analysis was used to examine the genetic diversity and structure of the IRRDB'81 germplasm. A total of 59 accessions from 13 different districts of(More)
An initial study on gene cloning and characterization of unicellular green microalga Ankistrodesmus convolutus was carried out to isolate and characterize the full-length cDNA of ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit (RbcS) as a first step towards elucidating the structure of A. convolutus RbcS gene. The full-length of A. convolutus(More)
The rapid and effective method for the isolation of RNA from green microalga Ankistrodesmus convolutus based on homogenization in a simple CTAB buffer and selective precipitation of RNA with lithium chloride is developed. This procedure avoids the use of toxic chaotropic agents and phenol while high concentration of dithiothreitol is used to inhibit RNase(More)
Isolation of promoter sequences from known gene sequences is a tedious task in genome-related research. An efficient method of obtaining the promoter sequences is necessary in order to successfully use targeted promoters for genetic manipulations. Here, efficiency and usefulness of two PCR-based methods, namely: ligation-mediated PCR and thermal asymmetric(More)
The structure of conventional engines is maintained while they are converted to biogaspowered engines so that they can reuse petroleum as fuel when needed as before conversion. In this case the detonation problem should be treated. This paper simulates the effects of compression ratio on the combustion in the engines fueled with biogas containing different(More)
1Department of Physics and Oxide Research Center, Hankuk University of Foreign Studies, Yongin 449-791, South Korea 2Faculty of Engineering Physics and Nanotechnology, VNU-University of Engineering and Technology, 144 Xuan Thuy, Cau Giay, Hanoi, Vietnam 3Department of Physics, Chungbuk National University, Cheongju 361-763, South Korea 4Institute of(More)
Germplasm collections have provided original materials for plant breeding program and crop improvement. Because of their genetic diversity and possible occurrence of particular desirable genes, germplasm collections are useful targets for plant breeders as well as other biologists. Recently, many of germplasm are being lost worldwide due to habitat(More)