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A simple method of separating cells according to their cellular densities is described. The distribution of pleural effusion cells of patients with pulmonary cancer was studied using Percoll discontinuous density gradients. Three density layers (1.050, 1.080 and 1.124 gm/ml) were used to separate the cells. After centrifugation (1,200 g for 20 minutes), the… (More)
Ceramics are often prepared with surface layers of different composition from the bulk, in order to impart a specific functionality to the surface or to act as a protective layer for the bulk material. Here we describe a general process by which functional surface layers with a nanometre-scale compositional gradient can be readily formed during the… (More)
Centrifugation into a rapid cell fixative is used to determine the conformation of red cells at various pH-values as a measure of red cell deformability. Discocytes show a good deformation into handbag-like forms; echinocytes and stomatocytes have reduced deformability with characteristic shapes of deformation.