Toshiki Mizobe

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Alpha2 adrenergic agonists are used in the anesthetic management of the surgical patient for their sedative/hypnotic properties although the alpha2 adrenoceptor subtype responsible for these anesthetic effects is not known. Using a gene-targeting strategy, it is possible to specifically reduce the expression of the individual adrenoceptors expressed in the(More)
The immunological manifestation of granuloma formations in humans largely depends on the delayed-type hypersensitivity response. We investigated the involvement of monocyte chemoattractant protein-1 (MCP-1) in a rabbit model of T cell-mediated pulmonary granulomatosis. Intravenous injection of Propionibacterium acnes (P. acnes) into sensitized rabbits(More)
AIMS 5-Fluorouracil (5-FU) is one of the most widely used anticancer drugs; however, the activity of 5-FU is determined by the presence of several enzymes that limit its activation or degradation, and these include dihydropyrimidine dehydrogenase (DPD), orotate phosphoribosyl transferase (OPRT), thymidylate synthase (TS), thymidine kinase (TK), thymidine(More)
In an attempt to investigate the subcellular trafficking of beta(2)-adrenergic receptor (beta(2)AR) in living cells, we performed real-time imaging of beta(2)AR tagged with green fluorescent protein (GFP). We transiently transfected a chimera construct of beta(2)AR and GFP (beta(2)AR-GFP) into HEK 293 cells, primary cultured rat hippocampal neurons and(More)
INTRODUCTION Venous catheterisation in paediatric patients can be technically challenging. We examined factors affecting catheterisation of invisible and impalpable peripheral veins in children and evaluated the best site for ultrasound-guided catheterisation. METHODS Systolic pressure, age, sex, and American Society of Anaesthesiologists (ASA) physical(More)
The 4th ventricle of a conscious rat was perfused using a push-pull cannula. The concentration of thyrotropin-releasing hormone (TRH) in the perfusate was significantly increased after withdrawal of 30% of the total blood. Administration of antiserum of TRH into the ventricle potentiated and prolonged the hypotension induced by the bleeding. These results(More)
Intracerebroventricular (i.c.v.) administration of thyrotropin-releasing hormone (TRH) caused an increase in blood pressure (BP) and heart rate (HR) in conscious rats. The pressor effect was greatly diminished by adrenalectomy as well as after pretreatment with phentolamine, an alpha-receptor antagonist or with mecamylamine, a ganglion blocker, suggesting(More)
The thyrotropin-releasing hormone (TRH) content in the brain was determined in normothermic and hypothermic rats subjected to immobilization stress. TRH contents in the hypothalamus, midbrain and cerebral cortex significantly decreased during mild hypothermia (body temperature about 34 degrees C), but not during profound hypothermia (about 24 degrees C).(More)
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