Toshikazu Tabata

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Primary graft nonfunction of steatotic liver allograft is one of the factors causing shortage of donor livers. Ischemia/reperfusion (I/R) injury is an important contributory factor to primary graft nonfunction. In this study, we investigated the complex chain of events from transcription factor activation to necrosis through cytokine induction and apoptosis(More)
We studied 75 BALB/c mice to examine the role of impaired immunoglobulin M (IgM) synthesis in the increased risk of bacterial infection after burn injury by investigating the kinetics of IgM synthesis to peptidoglycan polysaccharide (PGPS), a ubiquitous bacterial antigen. Splenocytes were isolated 1, 5, and 8 days postburn (PBD) and cultured with(More)
BALB/c mice (ischemia: 31; controls: 15) were studied to investigate the effects of intestinal ischemia on antibody synthesis to peptidoglycan polysaccharide (PGPS), a ubiquitous bacterial antigen found in both gram-positive and gram-negative bacteria. The gut ischemia model was produced by placing a vessel loop around the superior mesenteric vessels for 45(More)
Neutrophils have been identified to play a major role in ischemia/reperfusion injury through several mechanisms. Neutrophil migration into reperfused gut may reduce bacterial translocation, but may also enhance the reperfusion injury. Ethanol ingestion impairs cutaneous chemotaxis, but its effects on neutrophil migration to postischemic small bowel are(More)
In the presence of hydroxylamine or ascorbic acid, the inhibitory effects of Cu2+ on lysosomal acid cholesteryl ester hydrolase (acid CEH) partially purified from rat liver were studied. Hydroxylamine stimulated the inhibition of acid CEH activity by Cu2+ but not that by Zn2+, Fe2+, Co2+, Mn2+, Ca2+, Mg2+ and Hg2+. This Cu2+-dependent inhibition of acid(More)
The diurnal variation in lysosomal acid cholesteryl ester hydrolase (Acid CEH), (EC has been examined in fed, fasted and adrenalectomized rats. The Acid CEH activity of normal rat liver exhibits a diurnal rhythm with maxima at 06.00 hours and minima at 18.00 hours, but such a rhythm was not observed in spleen and brain. This rhythm was abolished(More)
The effect of chronic ethanol ingestion on class-specific immunoglobulin (Ig) synthesis after burn injury was investigated in C57BL/6 mice. Animals were divided into four groups: control, burn, ethanol-sham, and ethanol-burn groups. Five days after injury or the last ethanol ingestion, cell suspensions from spleen and mesenteric lymph nodes were prepared.(More)
The activity of lysosomal acid cholesteryl ester hydrolase (acid CEH, EC in rat liver was determined at 3, 5, 7, 10 and 20 wk following birth. The levels of acid CEH activity showed a marked decrease as rats grew older, whereas those of other lysosomal marker enzymes, such as acid phosphatase, β-glucuronidase and cathepsin B and D, showed only a(More)
Mice of the C57BL/6 strain were studied to investigate the effects of a 20% total body surface area full-thickness burn on class-specific immunoglobulin synthesis. Five days after injury single cell suspensions of spleen and mesenteric lymph node cells were made. The number of class-specific immunoglobulin-bearing cells were counted by flow cytometry using(More)
Hypolipidemic effects of gamma-oryzanol (OZ) and cycloartenol ferulic acid ester (CAF) on the hyperlipidemia induced by ingestion of a high cholesterol diet (HCD) in male Sprague-Dawley rats were investigated. The test drugs were given orally and intravenously, daily for 12 days with the HCD feeding. The oral administration with OZ and CAF at 100 mg/kg(More)