Toshihide Tsuzuki

Learn More
AIMS To assess immunohistochemically whether the neural cell adhesion molecule L1, which is a member of the immunoglobulin superfamily and has been shown recently to be a stimulating factor for glioma migration, is expressed in glioma tissues, and to investigate factors that can regulate this expression. METHODS Twenty seven glioma tissue specimens(More)
Alterations ofCDKN2A, RB, andcyclin D1 genes and expression of their products in astrocytic tumors were studied using a combination of molecular genetic and immunohistochemical assays. In addition, the association of gene status with clinical outcome was evaluated. Alterations ofCDKN2A andRB gene in 30 lesions were analyzed by single-strand conformation(More)
Restriction landmark genomic scanning (RLGS) is a 2-dimensionalgel analysis capable of detecting amplifications, deletions andrearrangements in genomic DNA. Using RLGS, we examinedgenomic DNA from each of 6 human-derived malignantglioma cell lines and from normal brain tissuesamples. RLGS allows us to screen genomic DNAsas approximately 2,000 landmark sites(More)
The 16 primary gliomas were examined for changes in genomic DNA using arecently developed 2-dimensional gel electrophoresis method calledrestriction landmark genomic scanning (RLGS). This approach allows detectionof DNA amplification, deletion, methylation and potentially other geneticrearrangements represented as decreases and increases in(More)
To cast light on the mechanisms of drug-resistance, experimental brain tumors were immunohistochemically evaluated for expression of glutathione S-transferase (GST)-α, µ, π, p-glycoprotein and apoptosis-related factors, such as bcl-2 and p53, as well as by the terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labelling (TUNEL) method. Rat(More)
Aims—To assess immunohistochemically whether the neural cell adhesion molecule L1, which is a member of the immunoglobulin superfamily and has been shown recently to be a stimulating factor for glioma migration, is expressed in glioma tissues, and to investigate factors that can regulate this expression. Methods—Twenty seven glioma tissue specimens(More)
The genetic mechanisms associated with recurrence of advanced astrocytic tumors are poorly understood. We therefore analyzed 24 biopsy specimens from 12 patients with a recurrent astrocytic tumor by a two-dimensional gel electrophoresis method, termed restriction landmark genomic scanning (RLGS). Four spot changes were commonly present in the primary(More)
Alterations of genomic DNA in eight primary astrocytic tumors and two glioma cell lines were examined using a recently developed two-dimensional gel electrophoresis method called restriction landmark genomic scanning (RLGS). RLGS allows us to detect amplifications, deletions, and methylation in genomic DNA in one procedure without requiring any polymorphic(More)
We present a case of symptomatic radiation necrosis after gamma knife surgery for arteriovenous malformations (AVMs). Initially, at the age of 30 years the patient was treated with gamma knife surgery. Angiography performed 2 years after radiation therapy revealed that the AVMs were completely obliterated. Five years later, the patient had a generalized(More)
  • 1