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Atomic details of near-transition state conformers for enzyme phosphoryl transfer revealed by MgF 3 - rather than by phosphoranes
Prior evidence supporting the direct observation of phosphorane intermediates in enzymatic phosphoryl transfer reactions was based on the interpretation of electron density corresponding to trigonalExpand
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Maximal expression of membrane-bound nitrate reductase in Paracoccus is induced by nitrate via a third FNR-like regulator named NarR.
Respiratory reduction of nitrate to nitrite is the first key step in the denitrification process that leads to nitrate loss from soils. In Paracoccus pantotrophus, the enzyme system that catalyzesExpand
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Two domains of a dual‐function NarK protein are required for nitrate uptake, the first step of denitrification in Paracoccus pantotrophus
Uptake of nitrate into the cytoplasm is the first but least well understood step of denitrification; no gene has previously been identified to be necessary for this process. Upstream from theExpand
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The denatured state under native conditions: a non-native-like collapsed state of N-PGK.
The guanidinium-denatured state of the N-domain of phosphoglycerate kinase (PGK) has been characterized using solution NMR. Rather than behaving as a homogenous ensemble of random coils, chemicalExpand
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A thiol labelling competition experiment as a probe for sidechain packing in the kinetic folding intermediate of N-PGK.
Protein folding is directed by the sequence of sidechains along the polypeptide backbone, but despite this the developement of sidechain interactions during folding is not well understood. Here, theExpand
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Measurement of the acute metabolic response to hypoxia in rat tumours in vivo using magnetic resonance spectroscopy and hyperpolarised pyruvate
Purpose To estimate the rate constant for pyruvate to lactate conversion in tumours in response to a hypoxic challenge, using hyperpolarised 13C1-pyruvate and magnetic resonance spectroscopy. MethodsExpand
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A system for accurate and automated injection of hyperpolarized substrate with minimal dead time and scalable volumes over a large range☆
Over recent years hyperpolarization by dissolution dynamic nuclear polarization has become an established technique for studying metabolism in vivo in animal models. Temporal signal plots obtainedExpand
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160 Modulation of Myocardial Ischaemic Injury by Carbon Monoxide Releasing Molecule-A1 in a Porcine Model of Acute Reperfusion Infarction
Introduction Conventional pharmacological treatments for acute myocardial infarction (AMI), a life threatening complication of a sudden coronary occlusion, are limited by their efficiency and sideExpand
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A relationship between hyperpolarized 13 C pyruvate metabolism and tumour volume in a chronic study of oral dichloroacetate treatment of P 22 bearing BDIX rat
tumour volume, mm3 kpl control kpl DCA A relationship between hyperpolarized C pyruvate metabolism and tumour volume in a chronic study of oral dichloroacetate treatment of P22 bearing BDIX ratExpand