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PURPOSE To investigate the function and pathogenicity of HRG4, a photoreceptor synaptic protein homologous to the Caenorhabditis elegans neuroprotein UNC119. METHODS HRG4 was screened for mutations in patients with various retinopathies, and a transgenic mouse model was constructed and analyzed based on a mutation found. RESULTS A heterozygous premature(More)
PURPOSE To characterize further HRG4, a novel photoreceptor protein recently identified by subtractive cDNA cloning, by sequence analysis and immunolocalization. METHODS The rat homolog of HRG4, RRG4 was expressed and used to prepare an antibody. The antibody was used in Western blot analysis, and immunofluorescent localization at the light and electron(More)
X-arrestin is a recently identified retina-specific gene of unknown function. Affinity-purified anti-peptide antibody to human X-arrestin was prepared, and used in Western blot analysis of human retinal proteins and for immunohistochemistry on human retinal sections. By Western blot analysis, the antibody specifically bound to an approximately 47 kDa(More)
PURPOSE Toll-like receptor 4 (TLR4) is a transmembrane receptor that mediates immune responses to exogenous and endogenous ligands and interacts with heat shock proteins, which are reportedly involved in normal tension glaucoma (NTG). This study was undertaken to investigate whether TLR4 polymorphisms are associated with NTG. METHODS Two hundred fifty(More)
A subtractive cDNA cloning strategy was used to isolate a 1381-base pair human retina-specific cDNA, human retinal gene 4 (HRG4), which hybridized to a 1.4-kilobase message in the retina and encoded a 240-amino acid acidic protein with a calculated molecular mass of 26,964 Da. The proximal 1/4 of the conceptual protein sequence was rich in glycine (18%) and(More)
PURPOSE To determine whether a scanning laser ophthalmoscope (SLO) is useful for in vivo imaging and counting of rat retinal ganglion cells (RGCs). METHODS RGCs of Brown Norway rats were retrogradely labeled bilaterally with the fluorescent dye 4-(4-(dihexadecylamino)styryl)-N-methylpyridinium iodine (DiA). The unilateral optic nerve was crushed(More)
HRG4 (UNC119) is a photoreceptor protein predominantly localized to the photoreceptor synapses and to the inner segments to a lesser degree. A heterozygous truncation mutation in HRG4 was found in a patient with late onset cone-rod dystrophy, and a transgenic (TG) mouse expressing the identical mutant protein developed late onset retinal degeneration,(More)
PURPOSE To evaluate long-term outcomes and identify prognostic factors of trabeculectomy following intraocular bevacizumab injection for neovascular glaucoma. METHODS Sixty-one eyes of 54 patients with neovascular glaucoma treated by trabeculectomy following intraocular bevacizumab injection were consecutively enrolled. Surgical success criteria were(More)
Among viscoelastic substances, sodium hyaluronate has become the most popular for intraocular surgeries since the introduction of Healon((R)) (sodium hyaluronate 1%, 4 x 10(6) daltons) in 1979. This review focuses on the recent development of a new generation of sodium hyaluronate agents with new rheologic properties and the relevant new techniques used in(More)
PURPOSE To investigate which measurements of inner macular thickness are the most useful for evaluating the focal relationship with visual sensitivity within the central 10° in glaucoma and which layers require correction for retinal ganglion cell (RGC) displacement. METHODS Sixty eyes of 60 subjects with glaucoma were included. Sensitivity of each test(More)