Tomoko Nagayama

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BACKGROUND Recent studies have revealed that the adiponectin-associated protein belonging to the C1qTNF family mediates various biological processes. However, the pathobiological property of C1qTNF6 in carcinogenesis remains unclear. Here, we investigated the expression status of C1qTNF6 in human hepatocellular carcinomas and subsequently attempted to(More)
Here, we report that the transcriptional regulator Zeb1 repressed the transcription of T-cadherin, to increase the invasive activity of gallbladder cancer cells. Zeb1 physically bound to the promoter of T-cadherin, repressed promoter activity in E-box-like sequence-dependent fashion, and suppressed T-cadherin expression. In gallbladder cancer tissues, Zeb1(More)
Using the PCR-based subtractive messenger RNA hybridization assay described in this paper, we isolated a hitherto uncharacterized gene, transmembrane protein 207 (TMEM207), which was selectively expressed in collagen gel-invading cultured signet-ring cell carcinoma KATO-III cells. TMEM207 has a C-terminal proline-rich PPxY motif, which binds to the WW(More)
The purpose of the present study was to examine the pathobiological properties of a matrix metalloproteinase, MMP-11 (also known as stromelysin-3), in the carcinogenesis of lobular carcinoma of the breast. Immunohistochemical staining demonstrated immunoreactivity with specific antibody to MMP-11 in 16 of 30 lobular carcinoma cells, but not in the(More)
The aim of this study was to examine whether Nedd4L (neural precursor cell expressed, developmentally down-regulated 4-like) participated in gallbladder carcinogenesis. We first immunohistochemically examined the expression of Nedd4L in various gallbladder tissue specimens. Weak immunoreactivity to Nedd4L-specific antibody was observed in normal or(More)
T-cadherin is believed to act against carcinogenesis in various tissues; however, its tumor-suppressor mechanism remains largely unclear. Using subtractive mRNA hybridization and immunoblotting, the present study identified several cancer-associated molecules whose expression was modified by T-cadherin in gallbladder cancer cells. Restoration of T-cadherin(More)
Fibroblast growth factor (FGF) signaling is involved in skeletal development. Among total 22 FGFs, it is suggested that FGF18 functions in promotion of osteoblast differentiation. In order to elucidate the mechanism of FGF18-dependent acceleration of osteogenesis, we implanted rhFGF18 soaked beads over mouse fetal coronal sutures using ex-utero surgery. The(More)
2,4-Dinitrotoluene (2,4-DNT) and its metabolites (2A4NT, 4A2NT, 2,4-DNB, 2A4NB, 4A2NB, 2,4-DAT, 2N4AAT, 2A4AAT, 2A4AABA and 2,4-DNBA), and 2,4-dinitrobenzaldehyde (2,4-DNA1), putative metabolite of 2,4-DNT, were tested for mutagenicity in Salmonella typhimurium strains TA98 and TA100. 2,4-DAT, 2N4AAT, 2A4AAT, 2A4AABA and 2,4-DNBA were inactive for strains(More)
We have established an approach to the isolation of expressed DNA sequences from a defined region of the human chromosome. The method relies on the direct screening of cDNA libraries using pooled single-copy microclones generated by a laser chromosome micro-dissection in conjunction with a single unique primer polymerase chain reaction (SUP-PCR) procedure.(More)