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To minimize the access cost in large disk arrays (RAID) Cohen et al. [5–7] introduced (d, f)-cluttered orderings of various set systems, d, f ∈ N. In case of a graph this amounts to an ordering of the edge set such that the number of points contained in any d consecutive edges is bounded by the number f. For the complete graph, Cohen et al. gave some… (More)

The gene encoding 2-oxo-hept-3-ene-1,7-dioic acid (OHED) hydratase (HpcG) was cloned into the high-expression plasmid pET26b and overexpressed in Escherichia coli BL21(DE3). The enzyme was purified in three steps to greater than 95% purity prior to crystallization. Crystals were obtained by the hanging-drop vapour-diffusion method at 277 K in a number of… (More)

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