Tomohiro Tobino

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Seawater-driven forward osmosis (FO) is considered to be a novel strategy to concentrate nutrients in treated municipal wastewater for further recovery as well as simultaneous discharge of highly purified wastewater into the sea with low cost. As a preliminary test, the performance of FO membranes in concentrating nutrients was investigated by both batch(More)
The goal of this study was to develop and validate a novel fosmid-clone-based metagenome isotope array approach - termed the community isotope array (CIArray) - for sensitive detection and identification of microorganisms assimilating a radiolabeled substrate within complex microbial communities. More specifically, a sample-specific CIArray was used to(More)
The use of randomly generated DNA fragment sequences as probes on DNA arrays offers a unique potential for exploring unsequenced microorganisms. In this study, the detection specificity was evaluated with respect to probe-target sequence similarity using genomic DNAs of four Pseudomonas strains. Genome fragments averaging 2000 bp were found to be specific(More)
This study reports on the development and application of a chronic sediment toxicity test using the benthic ostracod Heterocypris incongruens. H. incongruens reproduction parameters such as egg production, first day of brooding, egg-laying ratio, and hatching ratio were examined in this study. The test was first validated by determining the repeatability of(More)
The control of membrane fouling is still the biggest challenge that membrane bioreactor (MBR) for wastewater treatment faces with. In this report, we evince that an in-situ electrochemical free chlorine generation is effective for membrane fouling mitigation. An electrochemical oxidation (EO) apparatus with perforated Ti/IrO2 anodes and Ti/Pt cathodes was(More)
Inclined plate membrane bioreactors (ip-MBRs) have been proposed as a highly effective method in wastewater treatment. With the help of settling enhancer inclined plates, dense excess sludge can be kept in the mainstream of the process, and consequently, suitable sludge mass can be maintained in the membrane tank. In this study, the relationship among(More)
The shotgun isotope array method has been proposed to be an effective new tool for use in substrate-specific microbe exploration without any prior knowledge of the community composition. Proof of concept was demonstrated by detection of acetate-degrading microorganisms in activated sludge and further verified by independent stable isotope probing (SIP).
Biomarkers of exposure can be used to identify specific contaminants that are adversely affecting aquatic organisms. However, it remains prohibitively costly to investigate multiple novel biomarkers of exposure in a non-model species, despite the development of next-generation sequencing technology. In this study, we focused on the use of cDNA-amplified(More)
Urban road dust contains a variety of pollutants and might deteriorate the environment quality in the receiving water bodies of urban runoff. Urban road dust may be accumulated in the sediment for the periods of time, thus chronic toxicity should be evaluated. However, toxicity assessment of urban road dust is limited especially in the chronic effect on(More)
An acute sediment toxicity test using the ostracod Heterocypris incongruens is user friendly and has high sensitivity; however, a life-cycle test using this species has not been developed. The most challenging problem when developing a life-cycle test is that the egg development time varies greatly and is sometimes too long (> 150 d) to monitor. It is(More)