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BACKGROUND The objective of this prospective controlled trial was to investigate the ability of a group of serum and peritoneal fluid (PF) markers to predict, non-surgically, endometriosis. METHODS AND RESULTS Serum and PF samples were obtained from 130 women while undergoing laparoscopy for pain, infertility, tubal ligation or sterilization reversal.(More)
OBJECTIVE To test the feasibility of transplanting an intact frozen-thawed ovary with microvascular anastomosis of the ovarian vascular pedicle to the deep inferior epigastric vessels. DESIGN Chronic survival study. SETTING Biological Resources Unit, The Cleveland Clinic Foundation. ANIMAL(S) Adult merino ewes. INTERVENTION(S) Bilateral laparoscopic(More)
BACKGROUND The aim of this study was to assess the immediate post-thawing injury to the human ovary that was cryopreserved either as a whole with its vascular pedicle or as ovarian cortical strips. MATERIALS AND METHODS Bilateral oophorectomy was performed in two women (46 and 44 years old) undergoing vaginal hysterectomy and laparoscopic hysterectomy,(More)
OBJECTIVE To examine the presence of reactive oxygen species (ROS) in the follicular fluid of women undergoing in vitro fertilization (IVF) and identify its role in pregnancy outcome. STUDY DESIGN In this prospective study, ROS and total antioxidant capacity (TAC) levels were measured by the chemiluminescence method in the follicular fluid of 53 women.(More)
BACKGROUND Oxidative stress mechanisms are involved in the pathophysiology of many reproductive disorders. The objective of this study was to characterize oxidative stress parameters in hydrosalpingeal fluid (HSF) and examine their possible role in early embryo development. METHODS AND RESULTS HSF was aspirated at laparoscopic salpingectomy in 11(More)
OBJECTIVE To investigate the need for further surgery after laparoscopic excision of endometriosis or hysterectomy. METHODS In this retrospective study, women who had surgery for endometriosis-associated pain at the Cleveland Clinic were assessed for requirement for subsequent surgery. One hundred twenty patients who underwent hysterectomy with or without(More)
Many couples undergoing in-vitro fertilization (IVF) are at a higher risk of having a child with a genetic abnormality. In a sample of 55 consecutive couples starting IVF, only 33% had no genetic risk factor. The most common genetic risks were advanced maternal age and possible abnormalities associated with severe male infertility. Despite education on(More)
OBJECTIVE To evaluate the efficacy of reoperation for stage III or IV endometriosis-related infertility versus IVF-ET. DESIGN Retrospective analysis. SETTING In vitro fertilization-embryo transfer unit and tertiary infertility clinic. PATIENTS Twenty-three couples with stage III or IV endometriosis-related infertility undergoing IVF-ET and 18 women(More)
STUDY QUESTION What is the role of microRNA-451 (miR-451) in human endometriotic tissue? SUMMARY ANSWER miR451 expression was elevated in endometriotic lesion tissue. MiR451 modulated the expression of macrophage migration inhibitory factor and limited cell survival. WHAT IS KNOWN ALREADY microRNAs are post-transcriptional regulators of gene expression(More)
OBJECTIVE To evaluate the mitochondrial membrane potential (MMP) of spermatozoa and its correlation with semen parameters and production of reactive oxygen species (ROS) in infertile men and healthy donors. DESIGN Controlled prospective study. SETTING Male infertility clinic, Glickman Urological Institute, The Cleveland Clinic Foundation, Cleveland,(More)