Tom Kristensen

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No golden standard exists for normalization of real-time reverse transcriptase polymerase chain reaction (RT PCR) data and procedures used are often not validated. Numerous studies have indicated that current approaches are inadequate. Here, we report the development of an external RNA control approach. It is the first to add external RNA to tissue on a per(More)
Methane oxidizing prokaryotes in marine sediments are believed to function as a methane filter reducing the oceanic contribution to the global methane emission. In the anoxic parts of the sediments, oxidation of methane is accomplished by anaerobic methanotrophic archaea (ANME) living in syntrophy with sulphate reducing bacteria. This anaerobic oxidation of(More)
Cyanobacteria often produce several different oligopeptides, with unknown biological functions, by nonribosomal peptide synthetases (NRPS). Although some cyanobacterial NRPS gene cluster types are well described, the entire NRPS genomic content within a single cyanobacterial strain has never been investigated. Here we have combined a genome-wide analysis(More)
We have examined vector sequences fortuitously present in the EMBL sequence database as contaminating parts of submitted sequences, and found a sequencing error frequency of 3.55% in this subset of release 27 of the database. We discuss the possibility that this value may be representative for corresponding errors in the database as a whole.
A locus on human chromosome 16q22.1 contains at least five tightly clustered genes which are unrelated by sequence homology and apparently unrelated by function. The genes for a putative proteasome subunit (MECL 1), a chymotrypsin-like protease (CTRL), a protein serine kinase (PSKH1), the previously cloned lecithin:cholesterol acyl transferase (LCAT) and a(More)
The crucian carp, Carassius carassius, survives months without oxygen. During anoxia it needs to keep energy expenditure low, particularly in the brain, with its high rate of ATP use related to neuronal activity. This could be accomplished by reducing neuronal excitability through altered expression of genes involved in excitatory neurotransmission. Through(More)
The heterogeneity of distributed multimedia systems in terms of hardware, operating systems, programming languages, data formats, compression formats, available resources, and QoS requirements imposes severe problems. The purpose of the middleware platform is to provide interoperability and portability of distributed system services and applications.(More)
The task of middleware is to mask out problems of heterogeneity and distribution for application developers. With the emergence of new application domains, like multimedia and real-time applications, flexible support of QoS and real-time requirements becomes a major challenge. Reconfigurable bindings and policy driven binding protocols are the major means(More)
Two mutations are reported in six tyrosinemia type 1 patients from northern Europe. In four patients, a G to A transition at nucleotide position 1009 (G1009→A) of the fumarylacetoacetase (FAH) coding sequence caused aberrant splicing by introducing an acceptor splice site within exon 12, thereby deleting the first 50 nucleotides of this exon. The following(More)