Learn More
Isochrysis galbana, a marine prymnesiophyte microalga, is rich in long chain polyunsaturated fatty acids such as docosahexaenoic acid (C22:6n-3, Delta(4,7,10,13,16,19)). We used a polymerase chain reaction-based strategy to isolate a cDNA, designated IgASE1, encoding a polyunsaturated fatty acid-elongating activity from I. galbana. The coding region of 263(More)
We report the production of two very long chain polyunsaturated fatty acids, arachidonic acid (AA) and eicosapentaenoic acid (EPA), in substantial quantities in a higher plant. This was achieved using genes encoding enzymes participating in the omega3/6 Delta8 -desaturation biosynthetic pathways for the formation of C20 polyunsaturated fatty acids.(More)
Camptothecin (CN) is active against several experimental tumors and has also been studied clinically. We report here the effect of CN on LI210 cells and asynchronous and synchronous DON cells in culture. CN was toxic both to LI210 cells (0.06 ¿ig/ml,2-hr exposure) and DON cells (0.15 Mg/ml, 1-hr exposure), and it inhibited DNA and RNA synthesis more than(More)
Drug sensitivity and cell cycle parameters of L1210 cells in culture in exponential (10(5) to 4 x 10(5) cells/ml) and plateau phase (greater than 8 x 10(5) cells/ml) of growth were compared. The percentage of cells in G1, S, and G2 + M for exponential and plateau-phase cultures was 24.1, 70.0, and 5.9, and 42.5, 45.6, and 11.9, respectively. These values(More)
a control compound which killed cells in S but which did not affect the progression of cells into S. The cell-kill kinetics indicated that ara-C, HU, and 5-HP, unlike camptothecin, blocked the L1210 cells from progressing into S in the presence of the drug. We found that L1210 cells that were blocked in G¡by HU started moving into S immediately after the(More)
Twenty-six surgical breast cancer specimens were subjected to multiple estrogen receptor (ER) assays with a microsample technique. In most tumors there were large variations in ER levels in samples taken from different regions of the same tumor. After correction of individual samples for differences in carcinoma content, such variations were not abolished.(More)
5-Azacytidine (1 to 5 jug/ml) significantly inhibited mitosis of L1210 cells in culture only after 2 hr of incubation. The inhibition of mitosis was correlated to the inhibition of DNA synthesis. With asynchronous DON and LI210 cells, the cell-kill (inhibition of proliferative capacity) reached a saturation value at high doses of the drug. Any further(More)