Tomáš Gichner

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The heavy metal cadmium (Cd(2+)) applied on tobacco roots in the form of cadmium chloride, induced significantly higher levels of DNA damage as measured by the cellular Comet assay than did treatment of isolated root nuclei, analyzed by use of the acellular Comet assay. DNA damage induced by Cd(2+) in roots of a transgenic catalase-deficient tobacco line(More)
Tobacco (Nicotiana tabacum L. var. xanthi) seedlings were treated with aqueous solutions of lead nitrate (Pb2+) at concentrations ranging from 0.4 mM to 2.4 mM for 24 h and from 25 microM to 200 microM for 7 days. The DNA damage measured by the comet assay was high in the root nuclei, but in the leaf nuclei a slight but significant increase in DNA damage(More)
We employed single cell gel electrophoresis to analyze the kinetics of DNA repair in nuclei isolated from tobacco plants exposed to ethyl methanesulfonate (EMS), N-ethyl-N-nitrosourea (ENU) and gamma-radiation. DNA repair was measured as the reduction of the tail moment values as a function of time after the mutagen treatment ended. DNA damage in leaf(More)
The plant growth regulator and herbicide maleic hydrazide (MH) induced a high frequency of somatic mutations in leaves of tobacco (Nicotiana tabacum var. xanthi) and a high yield of chromosome aberrations in roots of field beans (Vicia faba, karyotype ACB). In contrast, no significant increase in MH-induced DNA damage, as measured by the Comet assay, could(More)
Plant tissue culture is now a modem technology for the in vitro production of a large number of genetically identical plants and has been commercialised for more than 10 years. Most of the companies in high labour cost areas have had to automate or to semi-automate their production system. This book presents a well-matched combination of articles dealing(More)
MASCARENHAS, J. P., BELT., E.: Protein synthesis during germination of pollen: studies on polyribosome synthesis. -Biochim. biophys. Acts 179 : 199--203, 1969. NITSC~, J. P., NITSCH, C.: Haploid plants from pollen grains. -Science 163 : 85--87, 1969. SATe, A., NODA, K., NATORI, Y.: The effect of protein depletion on the rate of protein synthesis in rat(More)
Heterezygous tobacco (Nicotiana tabacum var. xanthi) and potato (Solanum tuberosum var. Korela) plants were cultivated on soil from the site Strimice which is highly polluted with heavy metals and on nonpolluted soil from the recreational site Jezerí, both in North Bohemia, Czech Republic. The total content, the content of bioavailable, easily mobile, and(More)
We applied the alkaline version of the single-cell gel electrophoresis (comet) assay to roots and leaves of tobacco (Nicotiana tabacum var. xanthi) seedlings or isolated leaf nuclei treated with: (1) the alkylating agent ethyl methanesulphonate, (2) necrotic heat treatments at 50 degrees C, and (3) DNase-I. All three treatments induced a dose-dependent(More)
Tomáš Gichner a,∗, Zdeňka Patková a, Jiřina Száková b, Irena Žnidar c, Anita Mukherjee d a Institute of Experimental Botany, Academy of Sciences of Czech Republic, Na Karlovce 1a, 160 00 Prague 6, Czech Republic b Department of Agrochemistry and Plant Nutrition, Faculty of Agronomy, Czech University of Agriculture, 165 21 Prague 6-Suchdol, Czech Republic c(More)
The Annual Review of Genetics is published since 1967 by a non-profit scientific publisher. Thus the price of this volume is comparatively low compared to other similar publications. For copies of individual articles published in the Annual Review, or copies of any article cited in the Annual Review write to Annual Reviews Preprints and Reprints, P.OBox(More)