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Two techniques performed on skin biopsy samples (ear notches), immunohistochemistry (IHC) and antigen-capture ELISA (AgELISA), were compared for detection of bovine viral diarrhea virus (BVDV) persistent infection (PI) in 559 Angus calves between the ages of 1 and 5 months. The calves also were tested for BVDV infection using virus isolation (VI) and(More)
Scientists have feared that emerging infectious diseases could complicate efforts to conserve rare and endangered species, but quantifying impacts has proven difficult until now. We report unexpected impacts of West Nile virus (WNv) on radio-marked greater sage-grouse (Centrocercus urophasianus), a species that has declined 45–80% and is endangered in(More)
West Nile virus (WNV) resulted in a 25% decline in survival in four populations of radiomarked greater sage-grouse (Centrocercus urophasianus) across Alberta, Wyoming, and Montana in 2003. Unexpected impacts of WNV are disturbing because range-wide habitat loss and degradation already threaten sage-grouse populations. In the Powder River Basin of Wyoming(More)
Emerging infectious diseases pose a serious threat to wildlife conservation (Daszak et al. 2000), yet often little is known about consequences of emerging infectious diseases for populations of sensitive or declining native species. Evaluating impacts of emerging infectious diseases to wildlife populations is difficult because monitoring strategies rarely(More)
Understanding impacts of disease on wild bird populations requires knowing not only mortality rate following infection, but also the proportion of the population that is infected. Greater sage-grouse (Centrocercus urophasianus) in western North America are known to have a high mortality rate following infection with West Nile virus (WNv), but actual(More)
During February-April 2004, an estimated 400-500 free-ranging elk (Cervus elaphus) developed paresis, became recumbent, and died or were euthanized in the Red Rim Wildlife Habitat Management Area (RRWHMA), Wyoming, USA. Elk were found in sternal recumbency, alert and responsive, but unable to rise. Their condition progressed to lateral recumbency followed(More)
To determine if Mycobacterium avium paratuberculosis has persisted in tule elk (Cervus elaphus nannodes) at Point Reyes National Seashore (California, USA), 100 fresh fecal samples were collected. Feces were cultured on a modified BACTEC 12B radiometric medium for detection of M. avium paratuberculosis. Four samples, coming from two separate groups of elk(More)
Surveys for disease agents were conducted in introduced free-ranging elk (Cervus elaphus nelsoni) in Arkansas and Kentucky. Elk had been captured in Colorado and Nebraska and released in Arkansas during 1981-1985. From 1997 through 2002 elk were captured in Arizona, Kansas, North Dakota, New Mexico, Oregon, and Utah and released in southeastern Kentucky.(More)
The pathogenesis of vesicular stomatitis virus (VSV) infection has not been investigated previously in native New World rodents that may have a role in the epidemiology of the disease. In the present study, 45 juvenile and 80 adult deer mice (Peromyscus maniculatus) were inoculated intranasally with VSV New Jersey serotype (VSV-NJ) and examined sequentially(More)
Nine (four female, five male) captive adult Rocky Mountain bighorn sheep (Ovis canadensis) contracted brucellosis caused by Brucella abortus biovar 4 as a result of natural exposure to an aborted elk (Cervus elaphus) fetus. Clinical signs of infection were orchitis and epididymitis in males and lymphadenitis and placentitis with abortion in females. Gross(More)