Tina E Taub-Montemayor

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This paper compares the results of juvenile hormone (JH) titer determinations in two insect species, Melanoplus sanguinipes, a migratory grasshopper, and Acyrthosiphon pisum, the pea aphid, using a chiral-specific JH radioimmunoassay (RIA) and liquid chromatography tandem mass spectrometry (LC-MS/MS), after extraction of JH with either hexane or(More)
This report examines three aspects of adipokinetic hormone (AKH) involvement in migratory flight behavior in the grasshopper, Melanoplus sanguinipes. The titer of hemolymph AKH I during long-duration tethered flight was examined using radioimmunoassay (RIA) after narrow bore RP-HPLC. The hemolymph fraction containing AKH I was assayed using commercially(More)
Juvenile hormone (JH) is necessary for the production of vitellogenin (Vg) in the boll weevil, Anthonomus grandis. Occurrence of Vg in this species is typically restricted to reproductively competent females, and is not detected in untreated males. However, the JH analog, methoprene stimulates Vg production in intact males and in the isolated abdomens of(More)
Heinz Arnheiter Shalom Avraham John D. Baldwin Chris Burridge Donald G. Buth David Cannatella Michael T. Clegg John R. Demboski Rainer Duden Elias M. Elias Gary Garrett John R. Gold Thomas Henry James B. Holland Thomas P. Husband N. G. Jablonski Warren Johnson Praveen Karanth Nancy P. Keller Ian D. Krantz John Lacson Juan Lopez Lee McAlister-Henn C. Jane(More)
Reversed-phase high-performance liquid chromatography (RP-HPLC) allows for fractionation of extract from the corpora cardiaca of insects for examination of bioactivity or immunoreactivity and/or subsequent characterization of the peptides. Using RP-HPLC, we have previously identified an adipokinetic hormone (AKH) from the corpora cardiaca of the migratory(More)
A neuropeptide hormone isolated from corpora cardiaca of Melanoplus sanguinipes was purified by HPLC. The HPLC fractions were examined for adipokinetic activity with an in vivo bioassay. A single large UV absorbent peak was active in the mobilization of lipid while the other HPLC fractions showed no detectable activity. This large peak had a retention time(More)
The fluid dynamics of the reproductive system of the migratory grasshopper, Melanoplus sanguinipes F. (Orthoptera: Acrididae) was examined by the introduction of fluorescein-dextran (FD) into the hemocoel and observing its tissue specific sequestration. Male grasshoppers were observed to sequester FD first in the apical end of each sperm tube. FD then moved(More)
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