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Targeted activation of diverse CRISPR-Cas systems for mammalian genome editing via proximal CRISPR targeting
This work shows that the type II-B Fn Cas9 from Francisella novicida possesses novel properties, but its nuclease function is frequently inhibited at many genomic loci in living human cells, and develops a proximal CRISPR (termed proxy-CRISPR) targeting method that restores FnCas9 nucleasing activity in a target-specific manner.
CYP709B3, a cytochrome P450 monooxygenase gene involved in salt tolerance in Arabidopsis thaliana
It is suggested that CYP709B3 plays a role in ABA and salt stress response and provides evidence to support the functions of cytochrome P450 enzymes in plant stress response.
Improving CRISPR-Cas9 Genome Editing Efficiency by Fusion with Chromatin-Modulating Peptides.
Modification of the widely used Streptococcus pyogenes Cas9 by fusion with chromatin-modulating peptides (CMPs), derived from high mobility group proteins HMGN1 and HMGB1, histone H1, and chromatin remodeling complexes, improves its activity by up to several fold, particularly on refractory target sites.
Improving CRISPR Gene Editing Efficiency by Proximal dCas9 Targeting.
A proxy-CRISPR protocol for restoring nuclease activity of various class 2 CRISPR-Cas nucleases on otherwise inaccessible genomic sites in human cells via proximal targeting of a catalytically dead Cas9 ( Chen et al., 2017).