Timothy S. Gaginella

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Viable human T lymphoblasts derived from the "Molt 4b" cell line have been shown to possess functional plasma membrane receptors for vasoactive intestinal polypeptide (VIP). Specific binding of 125I-VIP to these lymphoblasts is rapid, reversible and linearly dependent on the number of cells present. Analysis of binding at 17 degrees C reveals a single class(More)
Vasoactive intestinal polypeptide (VIP) induces intestinal secretion of water and electrolytes in experimental animals and man. We assessed the ability of angiotensin II (AII) and norepinephrine (NE) to block the secretion evoked by VIP, in vivo. Ileal and colonic segments in rats were perfused in situ for two hours with a physiological buffer containing(More)
It is well established that bradykinin can stimulate mucosal electrolyte transport. However, the receptor type which mediates this effect has not been fully characterized. Recent studies have suggested that bradykinin and related kinins may act at two types of receptors designated as B1 and B2. We have determined the effect of bradykinin on electrolyte(More)
Release of [3H]norepinephrine ([3H]NE) from noradrenergic nerves innervating rat colonic mucosa was studied by superfusion of a muscle-stripped preparation of rat colon. After a period of incubation with [3H]NE, the tissue was superfused with different releasing agents. Depolarization of the tissue with a medium containing 50 mM K+ evoked the release of(More)
The release of vasoactive intestinal polypeptide (VIP) induced by electrical field stimulation (EFS) of rabbit ileum was studied in vitro. EFS parallel to the muscularis propria caused a significant increase in VIP concentration in the buffer bathing the serosal surface of full-thickness ileum. This effect was blocked by 10(-7) M tetrodotoxin. When circular(More)
We have examined the effect of caerulein on intestinal fluid transport in vivo. Rat jejunal, ileal, and colonic segments were perfused with a physiologic buffer for a 60-min control period, followed by a 60-min period after caerulein, prostaglandin E2 (PGE2), or saline (0.9%, w/v) were given by intramuscular (i.m) injection. At a dose of 5 micrograms/kg(More)