Timothy Perrine

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A modification of the apparatus previously described permits the preparation of cell walls in quantity. This consists of a heavy duty, double-acting hydraulic press with motor-driven pump, and a superstrength alloy steel pressure cell which is corrosion resistant. Liquid cooling of the jet is substituted for the previously used gas cooling to minimize(More)
Sedimentation and diffusion constants were determined for electrophoretically purified Vi antigen before and after mild alkaline hydrolysis. The molecular weight of the intact antigen was found to be 1.7 x 10(6). Mild alkaline hydrolysis completely removed O-acetyl, reduced the molecular weight to 1.0 x 10(5), and lowered the intrinsic viscosity from 8.0(More)