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We report the results of pyrosequencing of DNA collected from the activated sludge basin of a wastewater treatment plant in Charlotte, NC. Using the 454-FLX technology, we generated 378,601 sequences with an average read length of 250.4 bp. Running the 454 assembly algorithm over our sequences yielded very poor assembly, with only 0.3% of our sequences(More)
Changes in the composition of microbial communities are often examined using high-throughput sequencing (HTS). Here we analyzed bar-coded Illumina sequencing data from a previous work describing the microbial community found in the sputum of a cystic fibrosis patient by itself or in combination with qPCR to measure the absolute abundance of Pseudomonas(More)
Title ARISA data from the human gut microbiome can detect individual differences observed by 454 sequencing regardless of binning strategy. Abstract ARISA (Automated Ribosomal Intergenic Spacer Analysis) is a low-cost technique that allows for the rapid comparison of different microbial environments. In this study, we asked if a set of ARISA profiles can(More)
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