Timothy G. Keys

Publications14
h-index8
Citations159
Highly Influential Citations3
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Engineering the product profile of a polysialyltransferase.
Oligo- and polysaccharides have myriad applications as therapeutic reagents from glycoconjugate vaccines to matrices for tissue engineering. Polysaccharide length may vary over several orders of… Expand
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Cytoplasmic glycoengineering enables biosynthesis of nanoscale glycoprotein assemblies
Glycosylation of proteins profoundly impacts their physical and biological properties. Yet our ability to engineer novel glycoprotein structures remains limited. Established bacterial… Expand
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Screening a natural product-based combinatorial library using FTICR mass spectrometry.
This manuscript reports the use of Fourier transform ion cyclotron resonance mass spectrometry to screen a combinatorially generated natural product-based library for binding affinity to bovine… Expand
  • 27
A universal fluorescent acceptor for high-performance liquid chromatography analysis of pro- and eukaryotic polysialyltransferases.
Polysialyltransferases (polySTs) play critical roles in diverse biological processes, including neural development, tumorigenesis, and bacterial pathogenesis. Although the bacterial enzymes are… Expand
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A biosynthetic route for polysialylating proteins in Escherichia coli.
Polysialic acid (polySia) is a posttranslational modification found on only a handful of proteins in the central nervous and immune systems. The addition of polySia to therapeutic proteins improves… Expand
  • 17
A high-throughput screen for polysialyltransferase activity.
Polysialic acid is common to humans and a few bacterial pathogens and it holds great potential for the development of new therapeutic reagents. Currently, the bacterial polysialyltransferases… Expand
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A single amino acid toggles Escherichia coli polysialyltransferases between mono- and bifunctionality.
The enteropathogenic Escherichia coli K92 synthesizes a unique capsule consisting of polysialic acid (polySia) with alternating α2,8- and α2,9-linkages. The fact that a single enzyme is responsible… Expand
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Engineering protein glycosylation in prokaryotes
Abstract Controlling the specific glycan structure(s) present on a glycoprotein is a key challenge for both understanding its function and for developing effective next generation medical reagents. A… Expand
  • 17
Dissection of Hexosyl- and Sialyltransferase Domains in the Bifunctional Capsule Polymerases from Neisseria meningitidis W and Y Defines a New Sialyltransferase Family*
Background: Capsule polymerases of Neisseria meningitidis serogroups W and Y comprise hexosyl- and sialyltransferase activity. Results: Hexosyltransferase activity is encoded by the predicted… Expand
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Exploring and Exploiting Acceptor Preferences of the Human Polysialyltransferases as a Basis for an Inhibitor Screen
α2,8‐Linked polysialic acid (polySia) is an oncofoetal antigen with high abundance during embryonic development. It reappears in malignant tumours of neuroendocrine origin. Two polysialyltransferases… Expand
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