• Publications
  • Influence
Engineering the product profile of a polysialyltransferase.
Oligo- and polysaccharides have myriad applications as therapeutic reagents from glycoconjugate vaccines to matrices for tissue engineering. Polysaccharide length may vary over several orders ofExpand
  • 27
  • 2
Cytoplasmic glycoengineering enables biosynthesis of nanoscale glycoprotein assemblies
Glycosylation of proteins profoundly impacts their physical and biological properties. Yet our ability to engineer novel glycoprotein structures remains limited. Established bacterialExpand
  • 13
  • 1
Screening a natural product-based combinatorial library using FTICR mass spectrometry.
This manuscript reports the use of Fourier transform ion cyclotron resonance mass spectrometry to screen a combinatorially generated natural product-based library for binding affinity to bovineExpand
  • 27
A universal fluorescent acceptor for high-performance liquid chromatography analysis of pro- and eukaryotic polysialyltransferases.
Polysialyltransferases (polySTs) play critical roles in diverse biological processes, including neural development, tumorigenesis, and bacterial pathogenesis. Although the bacterial enzymes areExpand
  • 21
A biosynthetic route for polysialylating proteins in Escherichia coli.
Polysialic acid (polySia) is a posttranslational modification found on only a handful of proteins in the central nervous and immune systems. The addition of polySia to therapeutic proteins improvesExpand
  • 17
A high-throughput screen for polysialyltransferase activity.
Polysialic acid is common to humans and a few bacterial pathogens and it holds great potential for the development of new therapeutic reagents. Currently, the bacterial polysialyltransferasesExpand
  • 8
A single amino acid toggles Escherichia coli polysialyltransferases between mono- and bifunctionality.
The enteropathogenic Escherichia coli K92 synthesizes a unique capsule consisting of polysialic acid (polySia) with alternating α2,8- and α2,9-linkages. The fact that a single enzyme is responsibleExpand
  • 6
  • PDF
Engineering protein glycosylation in prokaryotes
Abstract Controlling the specific glycan structure(s) present on a glycoprotein is a key challenge for both understanding its function and for developing effective next generation medical reagents. AExpand
  • 17
Dissection of Hexosyl- and Sialyltransferase Domains in the Bifunctional Capsule Polymerases from Neisseria meningitidis W and Y Defines a New Sialyltransferase Family*
Background: Capsule polymerases of Neisseria meningitidis serogroups W and Y comprise hexosyl- and sialyltransferase activity. Results: Hexosyltransferase activity is encoded by the predictedExpand
  • 18
Exploring and Exploiting Acceptor Preferences of the Human Polysialyltransferases as a Basis for an Inhibitor Screen
α2,8‐Linked polysialic acid (polySia) is an oncofoetal antigen with high abundance during embryonic development. It reappears in malignant tumours of neuroendocrine origin. Two polysialyltransferasesExpand
  • 4