Basal forebrain (BF) neurons provide the principal cholinergic drive to the hippocampus and cortex. Their degeneration is associated with the cognitive defects of Alzheimer's disease. Immunohistochemical studies suggest that some of these neurons contain glutamate, so might also release it. To test this, we made microisland cultures of single BF neurons… (More)
The receptor subtype and transduction mechanisms involved in the regulation of various neuronal ionic currents are reviewed, with some recent observations on sympathetic neurons, hippocampal cell membranes and basal forebrain cells.
1. The actions of muscarinic agonists and antagonists upon the Ca2+ current (ICa) in acutely dissociated magnocellular cholinergic basal forebrain neurones from 11 to 14-day-old postnatal rats were studied using the whole-cell patch-clamp technique. 2. In all cells studied, muscarinic agonists inhibited a transient component of high-voltage-activated (HVA)… (More)
1. The electrophysiological and pharmacological characteristics of the calcium current (ICa) in acutely dissociated magnocellular cholinergic basal forebrain neurones from 11- to 14-day-old post-natal rats were studied using the whole-cell patch-clamp technique. 2. All cells exhibited a small transient low-voltage-activated (LVA) current with… (More)
1. Nicotinic acetylcholine (ACh) receptor-rich patches prepared from rat myotubes were used as focal ACh detectors to record the release of ACh from magnocellular basal forebrain (MBF) neurones from 11- to 14-day-old postnatal rats maintained in dissociated cell culture. 2. An action potential generated by intracellularly stimulating the MBF cell soma… (More)
The expression of ATP-sensitive K(+) (K(ATP)) channels by magnocellular cholinergic basal forebrain (BF) neurones was investigated in thin brain slice and dissociated cell culture preparations using a combination of whole-cell, perforated-patch and single-channel recording techniques. Greater than 95% of BF neurones expressed functional K(ATP) channels… (More)
Morphological and electrophysiological characteristics of magnocellular neurons from basal forebrain nuclei of postnatal rats (11-14 days old) were examined in dissociated cell culture. Neurons were maintained in culture for periods of 5-27 days, and 95% of magnocellular (>23 micron diam) neurons stained positive with acetylcholinesterase histochemistry.… (More)
Micro-island cultures provide a simplified system for studying the expression of cellular phenotype, excitability, synapse formation and pre- and postsynaptic regulatory mechanisms without the usual problems that arise from complex interactions between large numbers of other cells. The technique relies on the ability to constrain the attachment and growth… (More)