Timothy D. O’Connell

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Initial efforts of Alliance for Cellular Signaling (AfCS) laboratories have focused on establishing standard and reproducible procedures for purifying B lymphocytes (B cells) from mouse spleen. Our goal was to obtain cultures that permit reliable measurement of short-term signaling events and changes in gene expression. This report describes a(More)
Glycerol as a by-product of biodiesel production is an attractive precursor for producing d-glyceric acid. Here, we demonstrate the successful production of d-glyceric acid based on glycerol via glyceraldehyde in a two-step enzyme reaction with the FAD-dependent alditol oxidase from Streptomyces coelicolor A3(2). The hydrogen peroxide generated in the(More)
BACKGROUND Since volatile and rising cost factors such as energy, raw materials and market competitiveness have a significant impact on the economic efficiency of biotechnological bulk productions, industrial processes need to be steadily improved and optimized. Thereby the current production hosts can undergo various limitations. To overcome those(More)
␣1-Adrenergic receptor (AR) subtypes in the heart are expressed by myocytes but not by fibroblasts, a feature that distinguishes ␣1-ARs from ␤-ARs. Here we studied myocyte-specific expression of ␣1-ARs, focusing on the subtype ␣1C (also called ␣1A), a subtype implicated in cardiac hypertrophic signaling in rat models. We first cloned the mouse ␣1C-AR gene,(More)
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