Tim Tanigawa

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A gene for a pyrrolidone carboxyl peptidase (Pcp: EC 3.4.19.3, pyroglutamyl peptidase), which removes amino-terminal pyroglutamyl residues from peptides and proteins, has been cloned from the hyperthermophilic Archaeon Pyrococcus furiosus using its cosmid protein library, sequenced, and expressed in Escherichia coli. The DNA sequence encodes a protein(More)
High mobility group 2 (HMG2) protein is ubiquitously distributed in the nucleus of higher eukaryotic cells. Accumulation of an HMG2-beta-galactosidase fusion protein expressed in COS-7 cells suggested active transport of HMG2 protein into the nucleus after translation in the cytoplasm. Deletion analysis of the HMG2 sequence in the HMG2-beta-galactosidase(More)
Using colony formation in poly-L-lysine chamber slides and the polymerase chain reaction (PCR), we quantitated the number of human T lymphotropic virus type-I (HTLV-I) infected cells and proviral copies in peripheral blood lymphocytes from 25 pregnant HTLV-I carriers. The number of HTLV-I proviral copies in 0.25 microgram of DNA ranged from 2.8 to 9,176,(More)
There is a need to model complementary aspects of various data channels in distributed sensor networks in order to provide efficient tools of decision support in rapidly changing, dynamic real life scenarios. Our aim is to develop an autonomous cyber-sensing system that supports decision support based on the integration of information from diverse sensory(More)
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