Tianxi Zhang

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Being a non-renewable resource and a source of potential water pollution, phosphorus could be recovered from animal manure in the form of struvite (MgNH4PO4.6H2O) to be used as a slow-release fertilizer. It was found recently that the majority of phosphorus in anaerobically digested dairy effluent is tied up in a fine suspended calcium-phosphate solid, thus(More)
The monoacylglycerol lipase (MAGL) regulates 2-arachidonoyl glycerol (2-AG) level in the endocannabinoid system (ECS), which is implicated in a number of severe diseases such as cancer and Alzheimer’s disease. However, most existing MAGL inhibitors also show additional inhibitory effects on fatty acid amide hydrolase (FAAH), another member of the ECS that(More)
Nitrilases are an important class of industrial enzymes. They require mild reaction conditions and are highly efficient and environmentally friendly, so they are used to catalyze the synthesis of carboxylic acid from nitrile, a process considered superior to conventional chemical syntheses. Nitrilases should be immobilized to overcome difficulties in(More)
Lipases expressed in microbial hosts have great commercial value, but their applications are restricted by the high costs of production and harsh conditions used in industrial processes, such as high temperature and alkaline environment. In this study, an Escherichia coli–Bacillus subtilis shuttle vector (pHS-cotB-Tm1350) was constructed for the spore(More)
The present study describes the gene cloning, overexpression and characterization of a novel nitrilase from hyperthermophilic bacterium Thermotoga maritima MSB8. The nitrilase gene consisted of 804 base pairs, encoding a protein of 268 amino acid residues with a molecular mass of 30.07 kDa after SDS-PAGE analysis. The optimal temperature and pH of the(More)
Esterases expressed in microbial hosts are commercially valuable, but their applications are limited due to high costs of production and harsh industrial processes involved. In this study, the esterase-DSM (from Clostridium thermocellum) was expressed and successfully displayed on the spore surface, and the spore-associated esterase was confirmed by western(More)
In the present study, fusion genes composed of Thermotoga maritima MSB8 nitrilase and Bacillus subtilis 168 outer coat protein CotG were constructed with various peptide linkers and displayed on B. subtilis DB 403 spores. The successful display of CotG-nit fusion proteins on the spore surface of B. subtilis was verified by Western blot analysis and activity(More)
A gene coding for lipase (Tm1350) from the hyperthermophilic bacterium Thermotoga maritima MSB8 was cloned and overexpressed by Escherichia coli. The enzyme can degrade substrates with both short and long acyl chain lengths. The apparent Km and Vmax values for p-nitrophenyl butyrate were 8 mM and 333 U/mg, respectively. The enzyme displayed optimal activity(More)
This paper provides an empirical analysis of corporate governance (CG) efficiencies among Chinese listed companies (CLCs) under different types of ownership. Employing the data of over 700 CLCs in 2006, this study looks specifically into the effects of separation of controlling and residual claim rights upon CG (specifically management turnover) and firm(More)
In this work, a fantastic one-dimensional (1D) BiOBr/TiO2 nanorod (NR) heterojunction composite was rationally proposed and designed from the perspective of molecular and interface engineering. The fabricated intimately connected interfacial heterojunction between two-dimensional BiOBr nanoplates and 1D TiO2 NRs acts as an interfacial nanochannel to promote(More)