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A chimeric porcine circovirus type 1-2 (PCV1-2) infectious DNA clone has low transfection efficiency and exhibits low levels of proliferation. Electroporation and lipofection parameters were optimized for PK-15 and Dulac cells with the purpose of increasing the efficiency for rescuing infectious PCV1-2. Titers of PCV1-2 in Dulac cells were 100-fold higher(More)
Porcine circovirus type-2b (PCV2b) is recognized as the etiological agent of the various clinical manifestations of porcine circovirus-associated disease (PCVAD). Previous studies have demonstrated effectiveness of chimeric PCV1-2 vaccines against PCV2b challenge. In this study, the efficacy of inactivated and live-attenuated (2 × 103.5 or 2 × 104.0 50 %(More)
Since wireless sensor networks are usually used for long-term monitoring in harsh environments, sensor nodes are vulnerable to faults. Function fault can lead to immediate node breakdown, while data fault makes the node generate erroneous sensor data. Faulty data results in incorrect estimation of the environment and causes unnecessary consumption of the(More)
Porcine circovirus type 2 (PCV2) is subdivided into four genotypes: PCV2a, PCV2b, PCV2c and PCV2d. Here, for the first time, we compared the efficacy of two experimental inactivated chimeric PCV1-2 vaccines based on genotypes 2b and 2d. Seventeen 3-week-old pigs were divided randomly into four groups. Group 1 and 2 pigs were inoculated with genotype 2b- and(More)
Energy efficiency is one of the most critical issues in wireless sensor networks, since the sensor nodes are usually battery powered. These energy-constrained sensor nodes are usually densely distributed in indoor environments, which leads to spatially correlated sensor data and low network efficiency. Thus, one way to improve energy efficiency is to reduce(More)
Wireless sensor networks (WSNs) are widely considered as the most important information gathering platform in enabling Internet of Things (IoT). In order to evolve the traditional WSNs for low-power and low-loss IoT applications, time slotted channel hopping (TSCH) MAC protocol has been proposed to tackle the single channel and inefficient medium access(More)
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