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Leaf development in C4 plants such as maize involves the differentiation of two photosynthetic cell types [bundle sheath (BS) and mesophyll (M)] to form Kranz-type leaf anatomy. This cellular dimorphism partitions photosynthetic activities so that each enzyme of the C4 pathway accumulates only in the appropriate cell type. We have exploited this property to(More)
The spatial pattern of expression for a maize gene encoding a hydroxyproline-rich glycoprotein (HRGP) was determined by in situ hybridization. During normal development of roots and leaves, the expression of the gene was transient and particularly high in regions initiating vascular elements and associated sclerenchyma. Its expression was also associated(More)
C4 plants such as maize partition photosynthetic activities in two morphologically distinct cell types, bundle sheath (BS) and mesophyll (M), which lie as concentric layers around veins. We show that both light and cell position relative to veins influence C4 photosynthetic gene expression. A pattern of gene expression characteristic of C3 plants [ribulose(More)
We have established schedules of expression during maize leaf development in light and darkness for the messenger RNAs (mRNAs) and polypeptides for ribulose 1,5-bisphosphate carboxylase (RuBPCase) subunits, phosphoenolpyruvate carboxylase (PEPCase), and the light-harvesting chlorophyll a/b-binding protein (LHCP). Levels of mRNAs were measured by(More)
The regulation of members of the knotted1-like homeobox (knox) gene family is required for the normal initiation and development of lateral organs. The maize rough sheath2 (rs2) gene, which encodes a Myb-domain protein, is expressed in lateral organ primordia and their initials. Mutations in the rs2 gene permit ectopic expression of knox genes in leaf and(More)
It is suggested that ultrasound data acquisition will play an increasing role in the future of medical imaging. Unlike magnetic resonance imaging (MRI) and computerized tomography (CT), ultrasound offers interactive visualization of underlying anatomy in real time. Additionally, ultrasound equipment costs far less and does not use ionizing radiation or(More)
Chloroplast-localized NADP-dependent malic enzyme (EC (NADP-ME) provides a key activity for the carbon 4 fixation pathway. In maize, nuclear encoded NADP-ME is synthesized in the cytoplasm as a precursor with a transit peptide that is removed upon transport into the chloroplast stroma. We present here the complete nucleotide sequence for a(More)
Photosynthesis in C4-type grasses such as maize involves the interaction of two cell types (bundle sheath (BS) and mesophyll (M)) which both contain cell-specific photosynthetic enzymes. Malate dehydrogenase, phosphoenolpyruvate carboxylase and pyruvate phosphate dikinase are located in the M cells and malic enzyme and ribulose bisphosphate carboxylase are(More)
Development of the C4 photosynthetic pathway relies upon the cell-specific accumulation of photosynthetic enzymes. Although the molecular basis of this cell-specific gene expression is not known, regulation appears to be exerted at the level of transcript accumulation. We have investigated the relationship between gene expression patterns and DNA(More)
PURPOSE To prospectively and intraindividually compare dedicated breast computed tomographic (CT) images with screen-film mammograms. MATERIALS AND METHODS All patient studies were performed according to protocols approved by the institutional review board and Radiation Use Committee; informed consent was obtained. A breast CT scanner prototype was used(More)