Thomas N. Chiesl

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Functionalized nanoparticles hold great promise in realizing highly sensitive and selective biodetection. We report a single disposable chip which is capable of carrying out a multi-step process that employs nanoparticles--a bio-barcode assay (BCA) for single protein marker detection. To illustrate the capability of the system, we tested for the presence of(More)
Microfluidic laboratory-on-a-chip (LOC) systems based on a modular architecture are presented. The architecture is conceptualized on two levels: a single-chip level and a multiple-chip module (MCM) system level. At the individual chip level, a multilayer approach segregates components belonging to two fundamental categories: passive fluidic components(More)
We developed a two-layer, four-channel polymerase chain reaction (PCR)-capillary electrophoresis microdevice that integrates nucleic acid amplification, sample cleanup and concentration, capillary electrophoretic separation, and detection for multiplex analysis of four human respiratory viral pathogens, influenza A, influenza B, coronavirus OC43, and human(More)
An ultrasensitive nanoparticle-based biomolecule detection method, known as Bio-Bar-Coding (BBC) was recently developed by the Mirkin Group, which utilizes magnetic microparticle probes (MMP) functionalized with monoclonal antibodies that specifically bind to the target of interest [prostate-specific antigen (PSA) in this case; PSA is a prostate cancer(More)
We describe a novel class of DNA separation media for microchip electrophoresis, "physically cross-linked" block copolymer networks, which provide rapid (<4.5 min) and remarkably enhanced resolution of DNA in a size range critical for genotyping. Linear poly(acrylamide-co-dihexylacrylamide) (LPA-co-DHA) comprising as little as 0.13 mol % dihexylacrylamide(More)
We present a microfluidic system that allows for ultra-sensitive protein detection. The system integrates the bio-bar-code amplification (BCA) [1] approach with silver staining on a single microfluidic device to detect Prostate Specific Antigen in concentrations as low as 500 attomolar (300 proteins in the entire sample), using several orders of magnitude(More)
The Mars Organic Analyzer (MOA) has enabled the sensitive detection of amino acid and amine biomarkers in laboratory standards and in a variety of field sample tests. However, the MOA is challenged when samples are extremely acidic and saline or contain polyvalent cations. Here, we have optimized the MOA analysis, sample labeling, and sample dilution(More)
We demonstrate in this work the successful application of functionalized gold nanoparticles and magnetic microparticles in the development of an integrated lab-on-a-chip-system. By employing the BioBarCode Assay, we have shown that it is possible to quantitatively identify ultra-low concentrations of proteins not only in buffer solutions, but also in serum(More)
Presented in this paper is development of a protocol that employs microarrayed DNA patterns within the channels of an integrated lab-on-a-chip system. The patterned microchannels are used in conjunction with the Bio-Barcode Assay for the rapid multiplexed detection of six DNA disease biomarkers in a single experiment. The successful implementation of the(More)
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