Thomas M. Brennan

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Here, we describe assembly PCR as a method for the synthesis of long DNA sequences from large numbers of oligodeoxyribonucleotides (oligos). The method, which is derived from DNA shuffling [Stemmer, Nature 370 (1994a) 389-391], does not rely on DNA ligase but instead relies on DNA polymerase to build increasingly longer DNA fragments during the assembly(More)
Genetically expressed fluorescent proteins have been shown to provide photoacoustic contrast. However, they can be limited by low photoacoustic generation efficiency and low optical absorption at red and near infrared wavelengths, thus limiting their usefulness in mammalian small animal models. In addition, many fluorescent proteins exhibit low(More)
Signal offsets are a signal timing parameter that have a substantial impact on arterial travel times. The traditional technique is to optimize offsets using an offline software package, implement the settings, and then possibly observe field operations. It is not uncommon for a traffic engineer to fine tune the settings by observing the arrivals of platoons(More)
A wide variety of alternative optimization objective functions have been reported in the literature such as minimizing stops, minimizing delay, and maximizing arrivals on green. There is extensive literature evaluating these alternative objective functions using models. This paper reports on the field deployment of these alternative optimization functions,(More)
Familial hypomagnesemia with secondary hypocalcemia (HSH) (MIM 307600) was studied in three inbred Bedouin kindreds from Israel. The three kindreds, one extended and two nuclear families, contained 13 affected individuals, 11 males and two females. Assuming that the individuals affected with hypomagnesemia shared a chromosomal region inherited from a common(More)
Decisions on whether to coordinate adjacent intersections are currently made by rules of thumb, coupling indices based on ratios of volume to distance, and modeled traffic flows. As high resolution event data from signalized intersections becomes more readily available, it becomes possible to analyze actual link vehicle flows to better characterize whether(More)
ACKNOWLEDGMENTS This project has encompassed a number of studies in which many people have been involved. The authors of this project final report represent only a few of the individuals whose effort went into the overall endeavor. Therefore we would like to kindly thank the following individuals for their contributions to the work herein: The Joint(More)
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