Thomas M. Annesley

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BACKGROUND Mass spectrometry (MS) is being introduced into a large number of clinical laboratories. It provides specificity because of its ability to monitor selected mass ions, sensitivity because of the enhanced signal-to-noise ratio, and speed because it can help avoid the need for intensive sample cleanup and long analysis times. However, MS is not(More)
Mass spectrometry has long been used in clinical laboratories. The first important applications were the use of gas chromatography–mass spectrometry (GC-MS) for drug screening and the diagnosis of organic acid metabolic disorders. More recently liquid chromatog-raphy–mass spectrometry [LC-MS; liquid chromatog-raphy–tandem mass spectrometry (LC-MS/MS 2)] has(More)
We, the editors of the laboratory medicine journals, urge our colleagues in the International Committee of Medical Journal Editors (ICMJE) 1 to adopt and enforce the following requirements for clinical studies that include laboratory testing for biomarkers, and to add these statements to the information for authors: 1. For commercial diagnostic tests,(More)
BACKGROUND Matrix effects can profoundly reduce the performance of electrospray ionization mass spectrometry. Preliminary observations indicated that the methanol used in the mobile phase could be a source of differential ionization or ion suppression. METHODS Drug stability studies, analysis of biological extracts, mixing experiments, and postcolumn(More)
BACKGROUND The potent immunosuppressant mycophenolic acid (MPA) is metabolized to an inactive glucuronide (MPAG). The extent of metabolism varies among individuals, and the MPAG formed can be hydrolyzed to MPA and can displace MPA from serum albumin, creating a potential need to monitor both MPA and MPAG. METHODS After addition of the carboxybutoxy ether(More)
The Thomson Reuters (ISI) Web of Knowledge service lists data for nearly 7400 science journals. How many journals do you read on a regular basis? How many topics can you keep up with? If your answer to either question reached double digits, you are well beyond most scientists. The truth is that the number of papers published each year is so large that no(More)
You are nearing the end of the process of writing your scientific paper. You have carefully written a concise introduction, provided a detailed description of your methods, reported your results clearly, and discussed the meaning of your results. You may even have the perfect title and abstract ready to go. But the need to keep your focus and attention to(More)