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Global profiling of protein lipidation using chemical proteomic technologies
Highlights • Protein lipidation is an essential modification (PTM) in all forms of life.• Key modifications include acylation, prenylation, cholesterylation and GPI anchors.• Global profiling of thisExpand
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Dynamic Protein Acylation: New Substrates, Mechanisms, and Drug Targets.
Post-translational attachment of lipids to proteins is found in all organisms, and is important for many biological processes. Acylation with myristic and palmitic acids are among the most commonExpand
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Membrane bound O-acyltransferases and their inhibitors.
Since the identification of the membrane-bound O-acyltransferase (MBOATs) protein family in the early 2000s, three distinct members [porcupine (PORCN), hedgehog (Hh) acyltransferase (HHAT) andExpand
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Characterization of Hedgehog Acyltransferase Inhibitors Identifies a Small Molecule Probe for Hedgehog Signaling by Cancer Cells
The Sonic Hedgehog (Shh) signaling pathway plays a critical role during embryonic development and cancer progression. N-terminal palmitoylation of Shh by Hedgehog acyltransferase (Hhat) is essentialExpand
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Modulation of Amide Bond Rotamers in 5-Acyl-6,7-dihydrothieno[3,2-c]pyridines.
2-Substituted N-acyl-piperidine is a widespread and important structural motif, found in approximately 500 currently available structures, and present in nearly 30 pharmaceutically active compounds.Expand
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Competition-based, quantitative chemical proteomics in breast cancer cells identifies new target profiles for sulforaphane† †Electronic supplementary information (ESI) available: ProteomeXchange with
Protein targets of sulforaphane identified, and their affinities quantified, through competition-based chemical proteomics in two live breast cancer cell lines.
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Click chemistry armed enzyme-linked immunosorbent assay to measure palmitoylation by hedgehog acyltransferase
Hedgehog signaling is critical for correct embryogenesis and tissue development. However, on maturation, signaling is also found to be aberrantly activated in many cancers. Palmitoylation of theExpand
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Synthesis and characterisation of 5-acyl-6,7-dihydrothieno[3,2-c]pyridine inhibitors of Hedgehog acyltransferase
In this data article we describe synthetic and characterisation data for four members of the 5-acyl-6,7-dihydrothieno[3,2-c]pyridine (termed “RU-SKI”) class of inhibitors of Hedgehog acyltransferase,Expand
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Microfluidic Mobility Shift Assay for Real-Time Analysis of Peptide N-Palmitoylation
The Hedgehog pathway is a key developmental signaling pathway but is also implicated in many types of cancer. The extracellular signaling protein Sonic hedgehog (Shh) requires dual lipidation forExpand
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