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Pooled CRISPR screening with single-cell transcriptome read-out
CRISPR-based genetic screens are accelerating biological discovery, but current methods have inherent limitations. Widely used pooled screens are restricted to simple readouts including cellExpand
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Chromatin mapping and single-cell immune profiling define the temporal dynamics of ibrutinib response in CLL
The Bruton tyrosine kinase (BTK) inhibitor ibrutinib provides effective treatment for patients with chronic lymphocytic leukemia (CLL), despite extensive heterogeneity in this disease. To define theExpand
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Microbiota-Derived Metabolites Suppress Arthritis by Amplifying Aryl-Hydrocarbon Receptor Activation in Regulatory B Cells
Summary The differentiation of IL-10-producing regulatory B cells (Bregs) in response to gut-microbiota-derived signals supports the maintenance of tolerance. However, whether microbiota-derivedExpand
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Defining the microbial transcriptional response to colitis through integrated host and microbiome profiling
The gut microbiome is significantly altered in inflammatory bowel diseases, but the basis of these changes is not well understood. We have combined metagenomic and metatranscriptomic profiling of theExpand
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Single-cell transcriptomics combined with interstitial fluid proteomics defines cell-type-specific immune regulation in atopic dermatitis.
BACKGROUND Atopic dermatitis (AD) is the most common chronic inflammatory skin disease, but its complex pathogenesis is only insufficiently understood, resulting in still limited treatment options.Expand
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Circadian rhythm influences induction of trained immunity by BCG vaccination.
BACKGROUND The anti-tuberculosis vaccine Bacillus Calmette-Guérin (BCG) reduces overall infant mortality. Induction of innate immune memory, also termed trained immunity, contributes towardsExpand
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Structural cells are key regulators of organ-specific immune response
The mammalian immune system implements a remarkably effective set of mechanisms for fighting pathogens 1 . Expand
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Aryl Hydrocarbon Receptor Contributes to the Transcriptional Program of IL-10-Producing Regulatory B Cells
Summary Regulatory B cells (Bregs) play a critical role in the control of autoimmunity and inflammation. IL-10 production is the hallmark for the identification of Bregs. However, the molecularExpand
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Ultra-high throughput single-cell RNA sequencing by combinatorial fluidic indexing
We combine one-step combinatorial pre-indexing and droplet overloading to boost the throughput of droplet-based single-cell RNA sequencing using widely available droplet microfluidics. Expand
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The Transcription Factor MAZR/PATZ1 Regulates the Development of FOXP3+ Regulatory T Cells.
Forkhead box protein P3+ (FOXP3+) regulatory T cells (Treg cells) play a key role in maintaining tolerance and immune homeostasis. Here, we report that a T cell-specific deletion of the transcriptionExpand
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