Thomas Henriksson

Learn More
The amyloid forming beta-peptide of Alzheimer's disease is synthesized as part of a larger integral membrane precursor protein (beta APP) of which three alternatively spliced versions of 695, 751, and 770 amino acids have been described. A fourth beta APP form of 563 amino acids does not contain the beta-peptide region. Recent experiments using transient(More)
Rat liver mitochondria were subfractionated into outer membrane, intermembrane and mitoplast (inner membrane and matrix) fractions. Of the recovered protein kinase activity, 80-90% was found in the intermembrane fraction, while the rest was associated with mitoplasts. The intermembrane protein kinase was stimulated by cyclic AMP, while the mitoplast enzyme(More)
One of the major clinical findings in Alzheimer's disease (AD) is the formation of deposits of beta-amyloid protein in amyloid plaques, derived from the beta-amyloid precursor protein (beta-APP). To determine the possible use of beta-APP as a diagnostic marker for AD in CSF, a monoclonal antibody-based immunoassay specific for this protein was developed.(More)
Rat liver mitoplasts (inner mitochondrial membrane and matrix) contain protein kinase activity. This activity increases twofold on addition of Triton X-100. The activity observed in absence of Triton X-100 is probably exposed on the outer surface of mitoplasts, since it is sensitive to trypsin treatment. Most of the remaining protein kinase is bound to the(More)
Squalene is a naturally occurring oil which has been used in the development of vaccine adjuvants, such as the oil-in-water emulsion MF59. In past years, by use of noncontrolled and nonvalidated assays, a claim was made that antisqualene antibodies were detectable in the sera of individuals with the so-called Gulf War syndrome. Using a validated(More)
When rat liver plasma membranes were incubated with [gamma-32P]ATP radio-labelled phosphate was incorporated into endogenous protein and exogenous substrate by a membrane-bound protein kinase activity. A high ATP/membrane protein ratio was required for optimum incorporation conditions. Cyclic AMP did not affect the incorporation of phosphate. The protein(More)