Thomas H. Massey

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The assay of cerebrospinal fluid creatine kinase-BB (CK-BB) after cardiac arrest has demonstrated a relationship between CK-BB activity and neurologic recovery; a high concentration of cerebrospinal fluid CK-BB can be associated with lower Glasgow Coma Scale scores, intracranial pressure plateau waves, and histologic brain damage on death. Analysis of(More)
‘Zika fever’ was first reported in Uganda and Tanzania in 1952. Caused by the Zika flavivirus it consisted of a mild and self-limiting illness of rash, low-grade fever, conjunctivitis, headache and myalgia. Two strains of Zika virus were subsequently identified (African and Asian) with transmission via mosquito or sexual contact. Since 2007 there have been(More)
We studied the transient appearance of creatine kinase (EC 2.7.3.2) isoenzyme BB, as measured by electrophoresis, in serum or plasma from 19 patients who had just experienced cardiac or respiratory arrest. Creatine kinase BB activity was greatest 0.5 to 3 h after the arrest, with values (measured at 30 degrees C) ranging from 3 to 27 U/L (mean, 7.8 U/L) in(More)
Serum from patients who have suffered acute pancreatitis contains P3, an isoenzyme of pancreatic-derived amylase (EC 3.2.1.1). Heretofore, complete resolution of P3 from the major salivary isoenzyme in serum, S1, has not been possible, thus compromising the diagnostic potential of P3 for pancreatitis. I describe an electrophoretic method for the essentially(More)
Patterns of creatine kinase (CK, EC 2.7.3.2) isoenzymes were studied in apparently healthy one- to 10-day-old neonates, by use of a sensitive fluorescent staining method with Sclavo CK-F/6001 reagent. Mean activities of CK3 (MM, 105 U/L), CK2 (MB, 6.8 U/L), CK1 (BB, 11 U/L), adenylate kinase (EC 2.7.4.3) anodal to CK3, and a fluorescent albumin artifact(More)
A 36-year-old man presented to hospital with a 5-week history of ascending limb paraesthesiae and balance difficulties. He had no medical or travel history of note, but admitted habitual nitrous oxide (N2O) inhalation. Neurological examination revealed a sensory ataxia with pseudoathetosis in the upper limbs and reduced vibration sensation to the hips(More)
BACKGROUND AND PURPOSE Huntington's disease (HD) is an autosomal dominant, neurodegenerative movement disorder, typically characterized by chorea. Dystonia is also recognized as part of the HD motor phenotype, although little work detailing its prevalence, distribution, severity and impact on functional capacity has been published to date. METHODS(More)
We have adapted to a microcentrifugal analyzer an immunoinhibition assay for measuring the activity of creatine kinase MB by using an inhibitory antibody for the M monomer. The method actually measures half the MB activity, but results are not multiplied by two because atypical isoenzymes of creatine kinase, including BB, IgG-BB, and the isoenzyme derived(More)
In the decades since the genes and mutations associated with the commoner Mendelian disorders were first discovered, technological advances in genetic analysis have made finding genomic variation a much less onerous task. Recently, the global efforts to collect subjects with Mendelian disorders, to better define the disorders and to empower appropriate(More)
Antisense oligonucleotides (ASOs) are modified nucleic acids that alter gene expression in cells by binding target mRNA in a sequence-specific manner. ASO binding can trigger degradation or alter processing of mRNA. Potential advantages of this class of therapeutic include specificity (from base pairing), amplification of effect (one ASO can catalyse the(More)