Thomas F. Roth

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Quantitative and temporal features of maternal-fetal transport of IgG in the chicken have been determined by means of a sensitive and specific radioimmunoassay. The first step in this two-step process is the transport of maternal IgG from the hen plasma across the oolemma into the maturing oocyte in the ovarian follicle. Oocytes sequester IgG throughout(More)
Yolk proteins are thought to enter certain eggs by a process akin to micropinocytosis but the detailed mechanism has not been previously depicted. In this study the formation of protein yolk was investigated in the mosquito Aedes aegypti L. Ovaries were fixed in phosphate-buffered osmium tetroxide, for electron microscopy, before and at intervals after a(More)
Parasitic wasps constitute one of the largest group of venomous animals. Although some physiological effects of their venoms are well documented, relatively little is known at the molecular level on the protein composition of these secretions. To identify the majority of the venom proteins of the endoparasitoid wasp Chelonus inanitus (Hymenoptera:(More)
Polydnaviruses (PDVs) are unique symbiotic viruses associated with parasitic wasps; they replicate only in the calyx cells of a wasp's ovaries and are transferred at oviposition along with the parasitoid egg into the lepidopteran host. The DNA packaged in the viral particles encodes factors that manipulate the host's immune defences and development to(More)
Ferritin was purified from chicken liver by two different methods: gel filtration on controlled-pore glass beads, and immunoaffinity chromatography employing a chicken ferritin-specific monoclonal antibody that did not cross-react with horse spleen ferritin. This antibody recognizes intact ferritin and an oligomeric 240 kDa form of the molecule after(More)
In the chicken, maternal IgG is deposited in the oocyte yolk and subsequently transported intact by the foetal chick yolk sac into the chick circulation. Such transport appears to require a specific membrane-localized IgG receptor. Using the assay of 125I-yolk IgG binding, we have investigated the existence and properties of such receptors in yolk sac(More)
Survival of the chick depends upon the transfer of maternal IgG from the egg yolk into the fetal circulation. Radiolabeled chicken IgG binds specifically to both fixed and unfixed yolk sac tissue from day 7 or 8 of embryogenesis through at least day 18. This binding is saturable, Fc-specific, pH-dependent, and reversible. Chicken Fc fragments and some(More)
Acid-sensitive transferrin and albumin conjugates with doxorubicin have recently been developed with the aim of circumventing the systemic toxicity and improving the therapeutic efficacy of this anticancer agent. The in vitro activity of two acid-sensitive transferrin and albumin doxorubicin conjugates and free doxorubicin was evaluated in twelve human(More)
Eight-day-old dark-grown bean leaves were greened by prolonged irradiation with far red light. Growth, chlorophyll content, oxygen-evolving capacity, photophosphorylation capacity, chloroplast structure (by electron microscopy), and in vivo forms of chlorophyll (by low temperature absorption and derivative spectroscopy on intact leaves) were followed during(More)