Thomas D. Schmittgen

Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method.

The two most commonly used methods to analyze data from real-time, quantitative PCR experiments are absolute quantification and relative quantification. Absolute quantification determines the input… (More)

Analyzing real-time PCR data by the comparative CT method

Thomas D. Schmittgen, Kenneth J Livak
Nature Protocols
2008

Two different methods of presenting quantitative gene expression exist: absolute and relative quantification. Absolute quantification calculates the copy number of the gene usually by relating the… (More)

Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2 2 DD C T Method

Kenneth J Livak, Thomas D. Schmittgen
2001

The two most commonly used methods to analyze data from real-time, quantitative PCR experiments are absolute quantification and relative quantification. Absolute quantification determines the input… (More)

Effect of experimental treatment on housekeeping gene expression: validation by real-time, quantitative RT-PCR.

Thomas D. Schmittgen, Brian A Zakrajsek
Journal of biochemical and biophysical methods
2000

The effects of serum on the expression of four commonly used housekeeping genes were examined in serum-stimulated fibroblasts in order to validate the internal control genes for a quantitative RT-PCR… (More)

Involvement of human micro-RNA in growth and response to chemotherapy in human cholangiocarcinoma cell lines.

Fanyin Meng, Roger D Henson, +6 authors Tushar Patel
Gastroenterology
2006

BACKGROUND & AIMS Micro-RNA (miRNA) are endogenous regulatory RNA molecules that modulate gene expression. Alterations in miRNA expression can contribute to tumor growth by modulating the functional… (More)

Ultraconserved regions encoding ncRNAs are altered in human leukemias and carcinomas.

George A. Calin, Chang-gong Liu, +24 authors Carlo M. Croce
Cancer cell
2007

Noncoding RNA (ncRNA) transcripts are thought to be involved in human tumorigenesis. We report that a large fraction of genomic ultraconserved regions (UCRs) encode a particular set of ncRNAs whose… (More)

Detection of microRNA Expression in Human Peripheral Blood Microvesicles

Melissa Piper Hunter, Noura Ismail, +10 authors Clay B. Marsh
PloS one
2008

BACKGROUND MicroRNAs (miRNA) are small non-coding RNAs that regulate translation of mRNA and protein. Loss or enhanced expression of miRNAs is associated with several diseases, including cancer.… (More)

MicroRNA-34a inhibits glioblastoma growth by targeting multiple oncogenes.

Yunqing Li, Fadila Guessous, +11 authors Roger Abounader
Cancer research
2009

MicroRNA-34a (miR-34a) is a transcriptional target of p53 that is down-regulated in some cancer cell lines. We studied the expression, targets, and functional effects of miR-34a in brain tumor cells… (More)

Expression profiling identifies microRNA signature in pancreatic cancer.

Eun Joo Lee, Yuriy Gusev, +7 authors Thomas D. Schmittgen
International journal of cancer
2007

microRNAs are functional, 22 nt, noncoding RNAs that negatively regulate gene expression. Disturbance of microRNA expression may play a role in the initiation and progression of certain diseases. A… (More)

Real-time expression profiling of microRNA precursors in human cancer cell lines

Jinmai Jiang, Eun Joo Lee, Yuriy Gusev, Thomas D. Schmittgen
Nucleic acids research
2005

Our previous study described a real-time PCR method to quantify microRNA (miRNA) precursors using SYBR green detection [T. D. Schmittgen, J. Jiang, Q. Liu and L. Yang (2004) Nucleic Acids Res., 32,… (More)

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