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Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method.
The 2(-Delta Delta C(T)) method is a convenient way to analyze the relative changes in gene expression from real-time quantitative PCR experiments and two variations of the method are presented that may be useful in the analysis of real- time, quantitative PCR data.
Analyzing real-time PCR data by the comparative CT method
This protocol provides an overview of the comparative CT method for quantitative gene expression studies and various examples to present quantitative gene Expression data using this method.
Real-time quantitative PCR.
- Thomas D. Schmittgen
- 1 December 2001
Benefits of real-time PCR include enhanced sensitivity, high throughput, use of a closed-tube system, reduced variation, the ability to simultaneously multiplex reactions, and lack of post-PCR manipulations.
Effect of experimental treatment on housekeeping gene expression: validation by real-time, quantitative RT-PCR.
Involvement of human micro-RNA in growth and response to chemotherapy in human cholangiocarcinoma cell lines.
Altered in miRNA expression contribute to tumor growth and response to chemotherapy, and Aberrantly expressed miRNA or their targets will provide mechanistic insight and therapeutic targets for cholangiocarcinoma.
Detection of microRNA Expression in Human Peripheral Blood Microvesicles
This study is the first to identify and define miRNA expression in circulating plasma microvesicles of normal subjects, and provides a basis for future studies to determine the predictive role of peripheral blood miRNA signatures in human disease.
MicroRNA-34a inhibits glioblastoma growth by targeting multiple oncogenes.
It is suggested that miR-34a could serve as a potential therapeutic agent for brain tumors after being shown to suppresses brain tumor growth by targeting c-Met and Notch.
Expression profiling identifies microRNA signature in pancreatic cancer
- E. J. Lee, Y. Gusev, Thomas D. Schmittgen
- Biology, MedicineInternational journal of cancer
- 5 December 2006
A microRNA expression signature has been identified that is associated with pancreatic cancer and Aberrant microRNAs may offer new clues to pancreatic tumorigenesis and may provide diagnostic biomarkers for pancreatic adenocarcinoma.
Ultraconserved regions encoding ncRNAs are altered in human leukemias and carcinomas.
Quantitative reverse transcription-polymerase chain reaction to study mRNA decay: comparison of endpoint and real-time methods.
- Thomas D. Schmittgen, B. A. Zakrajsek, A. Mills, V. Gorn, M. J. Singer, M. Reed
- BiologyAnalytical biochemistry
- 15 October 2000
Four quantitative reverse transcription-PCR methods were compared to evaluate the time course of mRNA formation and decay and it was found that real-time PCR is more precise and displays a greater dynamic range than endpoint PCR.