Thomas Cech

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The L-2 1 ScaI ribozyme derived from the intervening sequence of Tetrahymena thermophila pre-rRNA catalyzes a guanosine-dependent endonuclease reaction that is analogous to the first step in self-splicing of this intervening sequence. We now describe pre-steady-state kinetic experiments, with sulfur substituting for the pro-Rp (nonbridging) phosphoryl(More)
Assays for Ribonucleolytic Activity 81 A sensitive and convenient assay for catalytic activity is an essential tool for studying catalysis. Because of the seminal role of bovine pancreatic ribonuclease A (RNase A; EC 3.1.27.5) in the history of enzymology, 1 assays for ribonucle-olytic activity have existed for decades. The recent discovery of ribonucleases(More)
Group I self-splicing introns catalyze their own excision from precursor RNAs by way of a two-step transesterification reaction. The catalytic core of these ribozymes is formed by two structural domains. The 2.8-angstrom crystal structure of one of these, the P4-P6 domain of the Tetrahymena thermophila intron, is described. In the 160-nucleotide domain, a(More)
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