• Publications
  • Influence
Two related pyrrolidinedione synthetase loci in Fusarium heterosporum ATCC 74349 produce divergent metabolites.
TLDR
Genetic mutation, biochemical analysis, and recombinant expression in the fungus enabled it to show that the initially assigned EqiS does not produce equisetin but instead produces a related 2,4-pyrrolidinedione, fusaridione A, that was previously unknown.
Isolation of Pyrrolocins A–C: cis- and trans-Decalin Tetramic Acid Antibiotics from an Endophytic Fungal-Derived Pathway
TLDR
The isolation, structure elucidation, and antimycobacterial activities of 1–3 from the recombinant expression as well as the isolation of 1 from the wild-type fungus NRRL 50135 are reported.
Identification of Cyclic Depsipeptides and Their Dedicated Synthetase from Hapsidospora irregularis.
TLDR
A comparison of the structures of leualacin and its analogues indicated that the A2 and A4 domains of the leUALacin synthetase are substrate specific, while A1, A3, and A5 can accept alternative precursors to yield new molecules.
Combinatorialization of fungal polyketide synthase-peptide synthetase hybrid proteins.
TLDR
The results of these experiments provide a set of ground rules for the successful engineering of hr-PKS and PKS-NRPS products in fungi, and show that C domains are highly selective for a subset of substrates within the highly reducing PKS class.
Native Promoter Strategy for High-Yielding Synthesis and Engineering of Fungal Secondary Metabolites
TLDR
The method resurrected an extinct pathway from an endophytic fungus with an initial yield of >800 mg L–1, leading to the practical synthesis of a selective antituberculosis agent and enabled new insights into the function of polyketide synthases in filamentous fungi.
Biosynthesis of the tetramic acids Sch210971 and Sch210972.
TLDR
Using genetic, biochemical, and feeding experiments, it is shown that the incorporated amino acid 4-hydroxyl-4-methyl glutamate (HMG) is synthesized by an aldolase, probably using pyruvate as the precursor.
An enzymatic Alder-ene reaction
TLDR
Two homologous groups of pericyclases that catalyze distinct reactions are discovered: one group catalyzes an Alder-ene reaction, previously unknown in biology; the second catalyzes a stereoselective hetero-Diels–Alder reaction.
Genome Mining of Alkaloidal Terpenoids from a Hybrid Terpene and Nonribosomal Peptide Biosynthetic Pathway.
TLDR
It is shown the flv pathway produces flavunoidine 1, an alkaloidal terpenoid, a tetracyclic, cage-like and oxygenated sesquiterpene that is connected to dimethylcadaverine via a C-N bond, and is acylated with 5,5-dimethyl-L-pipecolate.
Biosynthesis of para-Cyclophane-Containing Hirsutellone Family of Fungal Natural Products.
TLDR
The biosynthetic pathway for pyrrocidine B and GKK1032 A2 is elucidated and two small hypothetical proteins function cooperatively in the oxidative cyclization of the cyclophane.
Secondary Metabolites of Onygenales Fungi Exemplified by Aioliomyces pyridodomos.
TLDR
A large biosynthetic potential is delineated in Aioliomyces pyridodomos, the first representative of a candidate new family within Onygenales, which isolated 14 new bioactive metabolites and thirty-two specialized metabolite biosynthesis gene clusters were identified.
...
1
2
...