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Cryo-EM structure of substrate-bound human telomerase holoenzyme
The enzyme telomerase adds telomeric repeats to chromosome ends to balance the loss of telomeres during genome replication. Telomerase regulation has been implicated in cancer, other human diseases,Expand
CryoEM structure of the yeast U4/U6.U5 tri-snRNP at 3.7 Å resolution
U4/U6.U5 tri-snRNP represents a substantial part of the spliceosome before activation. A cryo-electron microscopy structure of Saccharomyces cerevisiae U4/U6.U5 tri-snRNP at 3.7 Å resolution led toExpand
The architecture of the spliceosomal U4/U6.U5 tri-snRNP
U4/U6.U5 tri-snRNP is a 1.5-megadalton pre-assembled spliceosomal complex comprising U5 small nuclear RNA (snRNA), extensively base-paired U4/U6 snRNAs and more than 30 proteins, including the keyExpand
Structural Basis of Brr2-Prp8 Interactions and Implications for U5 snRNP Biogenesis and the Spliceosome Active Site
Summary The U5 small nuclear ribonucleoprotein particle (snRNP) helicase Brr2 disrupts the U4/U6 small nuclear RNA (snRNA) duplex and allows U6 snRNA to engage in an intricate RNA network at theExpand
The cryo-electron microscopy structure of human transcription factor IIH
Human transcription factor IIH (TFIIH) is part of the general transcriptional machinery required by RNA polymerase II for the initiation of eukaryotic gene transcription. Composed of ten subunitsExpand
Structural studies of the spliceosome: zooming into the heart of the machine☆
Highlights • Structures of Prp8 and Brr2 have been determined.• Insights into the active site cavity of the spliceosome.• New evolutionary links between spliceosomes and group II introns.• Ligands ofExpand
Structural mechanism of ATP-independent transcription initiation by RNA polymerase I
Transcription initiation by RNA Polymerase I (Pol I) depends on the Core Factor (CF) complex to recognize the upstream promoter and assemble into a Pre-Initiation Complex (PIC). Here, we solve aExpand
Telomerase structures and regulation: shedding light on the chromosome end.
During genome replication, telomerase adds repeats to the ends of chromosomes to balance the loss of telomeric DNA. The regulation of telomerase activity is of medical relevance, as it has beenExpand
CryoEM structures of two spliceosomal complexes: starter and dessert at the spliceosome feast
Highlights • Recent advances in cryoEM are revolutionizing our understanding of how molecular machines function.• The structure of Saccharomyces cerevisiae U4/U6.U5 tri-snRNP has been revealed.• TheExpand
Generation of pseudocontact shifts in protein NMR spectra with a genetically encoded cobalt(II)-binding amino acid.
There is increasing interest in the paramagnetic labeling of proteins for structural studies by NMR spectroscopy. The resulting paramagnetic effects, particularly pseudocontact shifts (PCSs) andExpand