Theodoros Katsorchis

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Recombination in vaccine strains of polioviruses is a very frequent phenomenon. In the present report, 12 strains isolated from patients after OPV administration and healthy vaccinees were investigated for the identification of recombinant strains as well as for the further analysis of their recombination types and the localization of the recombination(More)
The 3D region of 46 clinical Coxsackievirus strains, primarily belonging to the human enterovirus B species (HEV-B), were analyzed using nucleotide distance matrices and phylogeny software. The conclusions from previously analyzed genomic regions (VP1—2A-2B-2C) of the aforementioned strains revealed that enteroviruses’ inheritance is being guided by gene(More)
Multiplex polymerase chain reaction (PCR) is a variant of PCR in which two or more target sequences are simultaneously amplified in the same reaction. In the present study we investigated the limits to which the duration of multiplex PCR steps can be shortened using the thermal cycler Gene Amp PCR system 9600 (Perkin Elmer, Oak Brook, IL). The present(More)
Modern molecular methods use VP1 coding region as a target for RT-PCR assays followed by sequencing, in order to identify new untyped enteroviruses’ strains. In the present study, two different genomic portions of VP1 and the full length of 2A coding region of 53 clinical isolates, mostly belonging to HEV-B species, were amplified and sequenced. Nucleotide(More)
Modern molecular approaches in Human Enterovirus detection rely on the designing of generic and often degenerate primers in order to amplify specific sequences within the enterovirus genome. In the present study a comparative application of primer sets targeting 5'UTR, the VP1 region, the 3D region as well as a long genomic fragment including the 3'end of(More)
Comparative studies of a gentamicin-sensitive strain (P28-0) of Pseudomonas aeruginosa and a gentamicin-resistant mutant (P23-800) have been carried out. No aminoglycoside-modifying enzymes were detected in extracts of the mutant. Electron microscopy of thin sections and the loss of O-antigenicity suggested that resistance of the mutant to gentamicin was(More)
In the present study, we demonstrate the isolation and characterization of the Pxd cDNA clone, which codes for the Drosophila melanogaster chorion peroxidase. This specific peroxidase is involved in the chorion hardening process, through protein crosslinking mediated by the formation of di- and tri-tyrosine bonds. The Pxd gene product has been identified in(More)
Modern molecular approaches on the genome of enteroviruses’ circulating strains have established new data about the mechanism and significance of its evolution. In the present study, 46 enteroviruses isolates, belonging to HEV-B species and exhibiting distinct origin in geographical or chronological terms, were investigated concerning their primary(More)