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Neutrophils contribute to inflammatory injury in some types of acute and chronic lung disease. Among the injurious agents released by activated neutrophils are cationic proteins such as cathepsin G that may contribute to edema formation. Using cultured rat type II alveolar epithelium, we examined the effect of human neutrophil cathepsin G on permeability to(More)
Type II alveolar epithelial cells play a crucial role in maintaining the structure and functions of pulmonary alveoli. A number of techniques have been described to isolate type II cells for in vitro studies; however, type II cell suspensions isolated by each technique are still contaminated by macrophages or monocytes. The present studies describe the use(More)
When type II alveolar cells are maintained in culture, they gradually lose their characteristic lamellar bodies and their ability to secrete surface active material. We evaluated the feasibility of using electron microscopy and stereology to quantitate the effects of multiple culture conditions on the ultrastructure of type II alveolar cells, in vitro. The(More)
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