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Rheumatoid arthritis (RA) has been thought to be largely a T-cell-mediated disease. To evaluate the role of T-cell-independent pathways in RA, we examined the interaction between isolated RA synovial fibroblasts and normal human cartilage engrafted into SCID mice in the absence of T cells and other human cells. The expression of cartilage-de grading enzymes(More)
BACKGROUND Vascular cell adhesion molecule-1 (VCAM-1) is expressed in synovial tissue of patients with rheumatoid arthritis. VCAM-1-protein has been demonstrated in nonvascular cells beside a vascular expression of this molecule. There are conflicting results about the nonvascular cell types expressing VCAM-1. EXPERIMENTAL DESIGN For the evaluation of(More)
OBJECTIVE A new animal model was used to study the interaction between rheumatoid synovial cells and cartilage and to explore the cellular basis of rheumatoid joint destruction. METHODS Fresh synovial tissue derived from patients with rheumatoid arthritis was implanted with normal human cartilage into SCID mice, either subcutaneously or under the renal(More)
OBJECTIVE To examine the de novo synthesis and cellular distribution of the E-selectin adhesion molecule in synovial tissues obtained from patients with rheumatoid arthritis (RA). METHODS Immunohistochemistry techniques combined with in situ hybridization were used to examine RA synovium. RESULTS There were numerous endothelial cells positive for(More)
Die rheumatoide Arthritis (RA) ist eine systemische Autoimmunerkrankung mit symmetrischer Gelenkentzündung zumeist peripherer Gelenke. Die Ätiologie der RA ist ungeklärt, den T-Zellen scheint aber dabei eine wichtige Rolle zuzukommen. Dagegen tritt die Psoriasis Arthritis (PsA) als asymmetrische Oligo- oder Polyarthritis auf, bei der distale(More)
Mit einem zunehmenden Kentnisstand über pathogenetische Mechanismen, die bei der rheumatoiden Arthritis zu einer Gelenkdestruktion führen, haben sich neue Therapieprinzipien entwickeln lassen, die durch eine Blockade von proinflammatorischen Zytokinen oder der Anwendung von antiinflammatorischen Zytokinen den Krankheitsverlauf zum Teil außerordentlich(More)
BACKGROUND A reliable, sensitive, and specific double labeling technique is required that allows the simultaneous visualization of in situ hybridization products and antigens. Currently used double labeling techniques are limited by various problems including the numerous disadvantages associated with radioactive labels, the time-dependent loss of(More)
OBJECTIVE To compare the expression of cathepsin L, cathepsin D, and collagenase messenger RNA (mRNA) in synovial specimens from patients with rheumatoid arthritis (RA) and osteoarthritis (OA). METHODS The expression of cathepsins L and D as well as collagenase mRNA in synovial tissues from 8 patients with RA, 6 patients with OA, and 2 patients with(More)