Throughout our investigations on the ontogenesis of the electrophysiological events in early embryonic chick hearts, using optical techniques to record membrane potential probed with voltage-sensitive dyes, we have introduced a novel concept of “functiogenesis” corresponding to “morphogenesis”. This article gives an account of the framework of… (More)
OBJECTIVE Mitochondrial respiratory chain disorder (MRCD) is an intractable disease of infants with variable clinical symptoms. Our goal was to identify the causative mutations in MRCD patients. METHODS The subjects were 90 children diagnosed with MRCD by enzyme assay. We analyzed whole mitochondrial DNA (mtDNA) sequences. A cybrid study was performed in… (More)
Multiple-site optical recording of membrane potential activity using a voltage-sensitive dye was employed to monitor action potentials in the rat atrium. Electrical pacing at 1 Hz evoked the events of tachycardia-like excitation (TE). Optical mapping revealed that a coupling of spontaneous and evoked action potentials resulted in the initiation of TE.