Teresa de la Rubia

Learn More
The fungus Phanerochaete flavido-alba is highly efficient in the oxidation of olive oil wastewater-derived polyphenols. This capability is largely due to the action of a multicopper-oxidase (MCO), encoded by the pfaL gene. We describe the sequence and organization of pfaL gene and the biochemical characterization and predicted 3D structural model of the(More)
Phanerochaete flavido-alba is able to remove simple and polymeric phenols from the recalcitrant wastes of the olive oil industry, in a process in which a laccase is involved. This report describes the characterization of a laccase produced byP. flavido-alba and induced by vanillin. Although the amino acid composition of the purified enzyme is typical for(More)
Growth and nitrogenase activity (acetylene reduction) of Azotobacter vinelandii in chemically defined N-free media were studied in the presence of p-hydroxybenzoic, vanillic, p-coumaric, and ferulic acids at concentrations from 0.01 to 1% (w/v). Growth and nitrogenase activity were only detected when the microorganism was cultured on p-hydroxybenzoic acid(More)
Inoculated and non-inoculated seedlings of Zea mays were grown in agricultural soils under aseptic and non-sterile conditions. Acetylene reduction activity and microbial counts were determined after 7 and 30 days of growth. Irrespective of the soil type Azotobacter spp. were commonly isolated under maize cultivation. Inoculation of agricultural soils with a(More)
Field experiments were conducted during 1985 and 1986 to examine the effect of Azotobacter chroococcum on the grain yield of maize. Application of 40 kg N ha−1 plus A. chroococcum caused a significant increase in maize yield. Azotobacter inoculation was more efficient at lower doses (40 kg N ha−1) than at high doses (80 kg N ha−1) of urea.
The effect of dicamba was studied in N-free medium inoculated withAzotobacter vinelandii ATCC 12837. Nitrogen fixation was determined by acetylene reduction. Dicamba at a concentration of 500 μg/mL had a strong inhibitory effect on nitrogenase activity. However, no inhibitory effect on microbial respiration was detected.
Our previous results have demonstrated that Phanerochaete flavido-alba decoloration, dephenolization and detoxification of olive oil mill wastewater (OMW) were associated with changes in the ligninolytic major exoenzymes accumulated in the cultures. This paper describes the effect of the two main OMW components (monomeric aromatic compounds and a major(More)
The effect of a low phosphate concentration on intracellular adenine nucleotide content, oxygen consumption and poly-β-hydroxybutyrate deposition was investigated with N-free and NH 4 + batch cultures of Azotobacter vinelandii. When the microorganisms were cultured under low-phosphate concentrations the cells contained much larger amounts of(More)
A soil organism, identified as Pseudomonas fluorescens var. aureofaciens was used to assay the phosphate content in defined liquid media. The organism showed a linear growth response to K2HPO4 from 0.6 mM to 6.10−6 mM. The organism did not grow at K2HPO4 levels below 6.10−6 mM and the response above 0.6 mM was not correlated with growth. It is suggested(More)
Batch cultures ofA. vinelandii in ammonium phosphate-limited and N-free phosphate-limited media were compared with control cultures (N-free phosphate-sufficient media). The effects of phosphate limitation on growth were determined by viable cells counts. Under phosphate-limitation conditions, growth inhibition and decreased viability were observed.(More)