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SUMMARY: A simple physical chemical method of transferring genes into eucaryotic cells is described. Electric impulses in the intensity range of 5-10 kV/cm with a duration of 5-10 bs were found to appreciably increase the uptake of DNA into cells. After electric field treatment, stable transformants were obtained in a system containing mouse cells deficient(More)
Peroxyacetic (PAA) and monoperoxydecanoic (MPDA) acids were assayed for induction of unscheduled DNA synthesis (UDS) by liquid scintillation counting of hot-acid-extractable DNA and by light microscope autoradiography. Both compounds were also assayed for induction of DNA repair synthesis by differential density labeling ultracentrifugation. Uniformly(More)
A granulomatous reaction to the small particles worn off from an implanted prosthesis is a common complication in arthroplasty. The chronic inflammatory response to the wear debris from either the polyethylene or the metal components is thought to be responsible for osteolysis, implant loosening, and finally mechanical failure. Formation of an(More)
A restriction fragment, 875 bp, which encodes for a beta-lactamase activity, was isolated from the Escherichia coli plasmid pBR322 DNA and entrapped in liposomes. The incubation of the DNA-liposomes with avian, murine, and human cultured cells results in the uptake of the DNA with the efficiency of around 2000 molecules per cell. Extracts of the recipient(More)
Pathogenicity of Meloidogyne hapla to lettuce was influenced by inoculum level, age of plant at inoculation and temperature. Top weight of 'Minetto' lettuce was reduced 32% when 2-week-old lettuce plants were each inoculated with five egg masses. Higher inoculum levels did not further decrease top weight significantly. Inoculation at seeding reduced top(More)
Evidence for the growth stimulatory properties of human keratinocyte-derived conditioned medium on human scalp dermal papilla cells and a mouse fibroblast cell line (Balb/c 3T3) is presented. There was no mitogenic effect on human keratinocytes. This conditioned medium induced a 2-5-fold increase in mitogenic activity in papilla cells relative to(More)
We present an improved method for the isolation and cultivation of human scalp anagen hair follicle dermal papilla cells. Following treatment of the isolated dermal papilla with collagenase, incubation in Chang's medium mediates accelerated growth of the papilla cells when compared with other media such as DMEM, M199, and EMEM. Upon reaching confluency, the(More)
The ability of sodium saccharin (NaS) to inhibit the repair of DNA damaged by UV irradiation was examined in cultured WI-38 human diploid fibroblasts. Cesium chloride density gradient ultracentrifugation was used to measure DNA repair and DNA replication. NaS (10-10,000 micrograms/ml) did not specifically inhibit UV light-induced DNA repair. At doses of NaS(More)