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Engineering of Halomonas bluephagenesis for low cost production of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) from glucose.
This is the first study to achieve the production of P(3HB-co-4HB) from only glucose using Halomonas, and 4HB molar fraction in the copolymer can be tuned from 13 mol% to 25  mol% by controlling the residual glucose concentration in the cultures.
Semirational Approach for Ultrahigh Poly(3-hydroxybutyrate) Accumulation in Escherichia coli by Combining One-Step Library Construction and High-Throughput Screening.
The results suggest that the semirational approach combining library design, construction, and proper screening is an efficient way to optimize PHB and other multienzyme pathways.
Novel T7-like expression systems used for Halomonas.
Several novel phage-derived expression systems used for transcriptional control in non- model bacteria are reported and envision these T7-like expression systems to benefit metabolic engineering in other non-model organisms.
Engineering of core promoter regions enables the construction of constitutive and inducible promoters in Halomonas sp.
The constitutive and inducible promoters were employed to regulate the biosynthetic pathway of poly‐3‐hydroxybutyrate (PHB) in Halomonas TD01, demonstrating the usefulness of the expression systems.
Pilot Scale-up of Poly(3-hydroxybutyrate-co-4-hydroxybutyrate) Production by Halomonas bluephagenesis via Cell Growth Adapted Optimization Process.
A stable and continuous open process for efficient low-cost production of P(3HB-co-4HB) is successfully developed coupling fermentation with the downstream extraction processing.
Fast multiple gene fragment ligation method based on Type IIs restriction enzyme DraIII
BBF RFC 61 is developed aimed at completing the ligation of multiple fragments quickly and efficiently based on Type II restriction enzyme DraIII at the recognition site of endonuclease DraIII.
Fast multiple gene fragment ligation method based on homologous recombination
This method depends on sequence and ligation-independent cloning (SLIC), which allows the assembly of multiple DNA fragments in a single reaction using in vitro homologous recombination and