Tatyana A Gureeva

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The prediction of biological activity spectra for substances as an approach for searching compounds with complex mechanisms of action was studied. New compounds with dual mechanisms of antihypertensive action were found by this approach. Biological activity spectra for substances were predicted on the basis of their structural formulas by the computer(More)
METHODS The activities of cathepsin L and its endogenous inhibitors were analyzed in rat embryo fibroblasts, immortalized and transformed by different genes. RESULTS Regardless of the transfecting agent used (DNA of adenovirus SA7 or polyomavirus LT gene), the immortal cells showed an increase in the cathepsin L activity (in both lysates and conditioned(More)
Dipeptidylpeptidase IV (DPP-IV) activity in lymphoid cells of patients with various lymphoproliferative diseases has been assayed. The enzyme activity was detected in lysates of all leukemic T- and B-cells studied. High DPP-IV activity levels (5-7 fold higher than those in mature Th and Ts) were found in T-cells with phenotypes corresponding either to the(More)
Fractionation of proteins of aqueous and salt (1 M KCl) extracts of human spleen on Sephadex G-100 revealed several proteinases hydrolyzing histone and casein and active in a neutral medium. The enzymes were found in the extracts in a relatively inactive form, due to the presence of an inhibitor, chiefly in the aqueous extract. Proteinases active in a(More)
Expression of matrix metalloproteinases (MMPs) and their endogenous regulators has been investigated in squamous cervical carcinoma (SCC). The study included (i) immortalized fibroblasts (IF) and three clones of fibroblasts transformed by oncogene E7 HPV-16 (TF); (ii) cell lines associated with HPV-16 and HPV-18; (iii) tumor tissue samples from patients(More)
Membrane type 1 matrix metalloproteinase (MT1MMP) is one of matrix metalloproteinases (MMP), which play а key role in tumor invasion and metastasis. The aim of this study was to elucidate the peculiarities of expression of MT1MMP and endogenous regulators of its activity: the activator - furin and the inhibitor - TIMP-2, as invasive factors of squamous cell(More)
Matrix metalloproteinases (MMP) play a critical role in tumor invasion and metastasis. The aim of this study was to elucidate peculiarity of expression of gelatinases A and B (MMP-2 and MMP-9), membrane type MMP (MT1-MMP) and tissue inhibitor of MMP (TIMP-2) in immortal (IF) and transformed fibroblasts (TF).The study was carried out using embryo rat(More)
Activities of dipeptidyl-aminopeptidase IV, urokinase-like plasminogen activator, cathepsins B and L were studied in lymphoid cells of patients with various forms of lymphoproliferative disorders. Activity of the enzymes studied was found in all the T- and B-cell, although rate and ratio of the enzymatic activity were dissimilar in various cell types. The(More)
A neutral SH-dependent proteinase was isolated from bovine spleen by a slight modification of the previous method (1) and its action on some natural and synthetic substrates was studied. The activity of the enzyme was increased 2000--2500-fold as compared to that of the original extract. The enzyme hydrolyzed various histones (H1, H2a, H2b, H3), casein and(More)
Many different kinds of tissue thiol proteinases, similar in properties to cathepsin B have been discovered recently [9]. Cathepsins H and L [9, i0], T [7], and M [12] have been isolated from the liver of rats and some other animals. Enzymes with similar properties, described as proteinases I and II [3, 4] and cathepsins N [6] and 8 [Ii], have been isolated(More)